“Bioprospection of Bergenia ciliata for Antioxidant, Antidiabetic, Hepato and Nephro Protective effects”
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Date
2018-04-24
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K)
Abstract
The studies on anti-diabetic, hepato-protective and nephro-protective potential of ethanolic extract of rhizome of B. ciliata were undertaken after determining therapeutically important phytochemical constituents and in vitro antioxidant potential of the extract of the plant. Total phenols, flavonoids, tannin, lycopene and β-carotene were found to be present in the ethanolic rhizome extract of the B. ciliata. Daily oral administration of the extract for 21 days in diabetic rats significantly attenuated altered mean blood glucose (MBG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), creatinine (CR), blood urea nitrogen (BUN), proteins, hepatic biomarkers (SGPT, SGOT, ALP and GGT) and antioxidant biomarkers (SOD, GST, G6PDH, MDA, TTH and GPx). Histopathological studies revealed regeneration of β –cells of islets of langerhans with daily administration of extract for 21 days. Such attenuating effect was superior after daily administration of 300mg/kg BW dose of extract as compared to lower dose of extract or decoction at either doses. Pre- and post-treatment with extract in acetaminophen induced hepatotoxicity in rats restored the elevated enzymatic hepatic biomarkers and altered antioxidant parameters in the blood as well as hepatic and renal tissues but such attenuation was found to be better at higher administered dose (300mg/kg BW). Histopathological studies also revealed higher administered dose restoring most of the hepatic cellular architecture excepting mild vacuolar degeneration. Pre- and post- treatment with plant extract at 300 mg/kg BW restored majority of the renal biomarkers in blood excepting total protein and globulin. Histopathological studies revealed both administered doses of extract failed to completely reverse the cDDP induced tubular nephrosis and glomerular congestion which indicates the poor nephroprotective potential of the extract of B. ciliata.
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