Development and characterization of transgenic pigeon pea (Cajanus cajan L. Millspaugh) plants with Psp68 for improvement against salinity stress
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Date
2019
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CCSHAU
Abstract
Pigeon pea (Cajnus cajan L.) is a nutrient rich, sixth most important and second most
important legume pulse crop of the world and India respectively after chickpea.Production and
productivity of pigeon pea is affected by various abiotic stresses but salt stress is the most deleterious
accounting for upto 100% yield losses. In the present study, transgenic pigeon pea plants (var. Manak)
carrying Psp68 gene were developed for salt stress tolerance using Agrobacterium tumefaciens strain
LBA4404 with pCAMBIA1300-Psp68 (Kharb et al., 2018). Putative transformants were screened at
early stage through PCR amplification using gene specific primers and a transformation frequency of
16% was observed. Southern blot and real time PCR analysis revealed stable and single copy insertion
of the transgene in pigeon pea genome. Seeds from each of the PCR positive T0 pigeon pea plants
were sown to raise T1 generation and a total of 42 plants (out of 100) showed the presence of Psp68
gene. Physio-biochemical analysis of selected T1 transgenic plants subjected to 75 mM salt stress
showed that transgenic plants were able to maintain higher chlorophyll content, relative water content,
proline content, total soluble sugars, catalase and peroxidase activity compared to the wild type plants.
Whereas, electrolytic leakage and lipid peroxidation were reduced as compared to the wild type plants
under 75 mM stress. Among all transgenic lines, line 53 performed well with respect to all the
parameters studied and can be taken further for the development of transgenic pigeon pea plants for salt
stress tolerance.
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