Cultural and Metagenomic Characterization of Lower Respiratory Microbiome of Sheep
Loading...
Date
2018-07-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K)
Abstract
Sheep is an important species of livestock that has an immense potential to provide livelihood to a large proportion of small and marginal landless farmers, but respiratory diseases are one of the major hurdles in development of this sector. This study was conducted to explore the microbial diversity in lower respiratory tract of healthy as well as diseased sheep using conventional culture based and metagenomic approach using Illumina miseq platform. In cultural analysis a total of 23 bacterial isolates (8 from healthy and 15 from diseased) were found belonging to 4 Phyla, 7 orders, 8 families, 15 genera and 19 species. However, in metagenomic study the 16S rRNA sequences showed high diversity of reads corresponding to, 8 phyla, 21 classes, 38 order, 62 families, 76 genera, and 43 species in healthy sheep. While in diseased sheep the diversity of reads corresponds to 11 phyla, 22 classes, 51 order, 93 families, 105 genera, and 97 species in diseased sheep. Out of these taxanomic units at phylum level proteobacteria was found to be the most abundant in both healthy as well as in diseased sheep (41.15 % in healthy, 37.54% in diseased), while at order level Pseudomonadales were abundant in both healthy and diseased sheep (61.59% in healthy, 47.12% in diseased). At class level Gamma-proteobacteria was abundant in healthy sheep (79.29%) whereas Actinobacteria (61.23%) was abundant in diseased sheep. At family level Moraxellaceae(69.83%) was abundant in healthy and Sphingomonadaceae(54.12%) was the most abundant in diseased sheep. At genus level Acinetobacter (69.75%) was most abundant in healthy sheep while Brevundimonas (63.25%) was the most abundant in diseased sheep. At species level Uncultured Acinetobacter was most abundant (69.54%) in healthy sheep and Pasteurella multocida (37.15%) was the most abundant in diseased sheep. The study revealed presence of a rich and very diverse microbial flora in the lower respiratory tract of sheep and is probably the first comprehensive study on sheep respiratory tract in India that can serve as a benchmark for further research in respiratory diseases of sheep.
Description
Cultural and Metagenomic Characterization of Lower Respiratory Microbiome of Sheep
Keywords
null