Effect of dietary cholesterol and niacin supplementation on experimentally induced hepatic cirrhosis in wistar rat
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Date
2018-07-20
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (J&K)
Abstract
The present study was undertaken to evaluate the effect of dietary cholesterol and niacin supplementation and hepatic cirrhosis in wistar rats. 84adult healthy wistar male rats were divided into twelve groups. Experiment was carried out as per 2 x 3 x 2 factorial design with two clinical conditions (healthy rats and rats with hepatic cirrhosis); three levels of cholesterol supplementation (0%, 0.5% and 1.0%) and two niacin levels (unsupplemented and supplemented @ 100mg/kg body weight), respectively. At the start of the experiment, hepatic cirrhosis was induced in rats of cirrhotic groups by intra-peritoneal injection of carbon tetrachloride @ 0.5ml/kg body weight dissolved in olive oil (1:1 ratio) for 21 days alternatively. Blood samples were collected periodically and analysed for biochemical parameters and antioxidant profile. Hepatic glycogen, hepatic oxidative stress, immune response and semen quality were also assessed. At the end of trial, animals were sacrificed and liver, kidney, spleen and testes were harvested for histopathological analysis. Hepatic cirrhosis and cholesterol supplementation resulted in significant augmentation of almost all the biochemical, enzymatic, oxidative stress, immune and semen quality parameters with significant interaction among these two factors. Meanwhile, niacin supplementation had a favourable effect over these parameters and was also able to mitigate the adverse impact of hepatic cirrhosis and/or dietary cholesterol supplementation with significant interaction. It can be concluded that niacin supplementation appears to have hepatoprotective, nephroprotective and antioxidant effect under experimentally induced hepatic cirrhosis in hypercholesterolemic wistar rats.
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Effect of dietary cholesterol and niacin supplementation on experimentally induced hepatic cirrhosis in wistar rat
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