SEED HEALTH EVALUATION OF DIFFERENT VARIETIES OF INDIAN BEAN (Lablab purpureus L.)
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Date
2019
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
The present investigation was carried out in the seed pathology lab of Department of Plant Pathology, College of Agriculture, IGKV, Raipur (C.G.) during 2018-19. Seed health evaluation was attempted for seed lots of different Indian bean varieties viz. IS-1, IS-2, IS-12, IS-29, IS-96 and local variety from randomly selected village. In dry seed examination, seed lots of different Indian bean varieties showed distinct variation in healthy seeds, damaged, small/under sized, shrunken seeds, weed seeds and inert matters. Maximum spore load of associated seed borne mycoflora was observed in seed lot of local variety by following washing test. Thirteen seed borne mycoflora viz. Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Alternaria alternata, Botryosporium sp. Curvularia lunata, Chaetomium globosum, Fusarium sp., Nigrospora sp., Penicillium sp., Pithomyces sp., Rhizopus stolonifer and Trichoderma viride were detected and identified from the seeds of six Indian bean varieties by using standard blotter, agar plate, rolled paper towel, deep freeze and component plating method. Maximum percentage of mycoflora with reduced germination percentage was recorded from the seed lots of local variety whereas IS-29 variety harbours minimum percentage of mycoflora with higher germination percentage. Seedling symptom test was also performed by water agar test tube method and found the normal intact seedlings from the seeds inoculated with Trichoderma viride and Penicillium sp., attributing the plant growth promoting properties. Agar plate method was most efficient in detecting seed borne mycoflora among all other incubation methods. Effect of isolated seed borne fungi was also tested for seedling vigour under seedling symptoms in pot culture condition by using seed and soil inoculation techniques. Majority of the detected seed borne mycoflora reduced the seedling vigour index and some were not. Two seed borne mycoflora of Indian bean seeds i.e. Aspergillus niger and Curvularia lunata were found pathogenic and seed to plant transmissible in nature whereas increased seedling vigour of Indian bean varieties were recorded due to growth promoting ability of Trichoderma viride. Seed lots were primed with fungicides and observed increased seedling vigour index as compare to untreated ones. Seed lots were also bioprimed with phytoextracts and observed increased seedling vigour index as compare to untreated ones. After six months storage of seed lots, electrolyte leakage of Indian bean seeds were recorded in terms of electrical conductivity and pH value of seed leachate. Due to the presence of seed borne mycoflora the EC value was increased and corresponding pH values were found inversely proportion.
Description
SEED HEALTH EVALUATION OF DIFFERENT VARIETIES OF INDIAN BEAN (Lablab purpureus L.)
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