STABILITY ANALYSIS AND SCREENING FOR SHOOT FLY TOLERANCE IN ADVANCED BREEDING LINES OF SORGHUM (Sorghum bicolor (L.) Moench)

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Date
2014-06-30
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UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
Abstract
An experiment was carried out involving twenty three advanced breeding lines of sorghum along with two checks M 35-1 and Muguthi during Rabi season 2012-13 at four different locations viz., Agriculture Research station Gulbarga, Main Agriculture Research station Raichur, Agriculture Research station Malnoor and Agriculture Research station Hagari. The genotypes also assessed for, shootfly tolerance screening (kharif and rabi-2013) and validation of SSR marker for shootfly resistance traits. The pooled analysis of variance revealed that mean sum of squares due to genotypes was significant for most of the characters, indicating the considerable amount of variability in the material chosen for the study. The mean sum of square due to environment + (genotypes x environment) was significant for most of the characters except stem diameter, days to maturity and 100 seed yield. On the basis of stability parameters the few genotypes viz., GS-6, GS-16, GS-22 and GS-23 were found most stable for majority of the characters over all four environments. Correlation coefficient revealed that plant height, ear head length, days to maturity, 100 seed weight, fodder yield and lodging percentage were positive correlation with seed yield per plot over environments at genotypically and phenotypically. Path analysis revealed the positive direct effect of plant height, ear head length, ear head diameter, days to 50 per cent flowering, fodder yield and lodging percentage at phenotypic and genotypic level. Screening for shoot fly resistance with interland fish-meal technique at both kharif and rabi seasons. Genotypes GS-2, GS-3, GS-5, GS-8, GS-11 and GS-22 showed moderately resistance at kharif season. Whereas, GS-1 shows moderately resistance at rabi season. Validations of molecular markers reveals that marker and trait association was non-significant due to the selected markers were QTL flanking markers, however it may be fluctuate due to environmental influence and large genome size.
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