COMPARISON OF VARIOUS METHODS OF ISOLATION AND IDENTIFICATION OF YERSINIA SPECIES IN MILK AND MILK PRODUCTS
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Date
2010
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Abstract
On biochemical characterization of suspected Yersinia species isolated from milk and milk
products, twenty (l9.42%) were identified as Yersinia sp. by the conventional biochemical methods
which include 9 Yersinia enterocolitica, 6 Y. frederiksenii and S Y. kfisrensenii where as, 18 by the
commercial kit method which include 7 Y. emerocolitica, 6 Y frederiksenii and 5 Y kriszensenii
Among the 29 presumptive Yersinia isolates tested in PCR assay, only nine were identified as Y
entrrocolirim, as they showed amplification for species specific 16S rRNA gene (330 bp). In multiplex
PCR, the same 4 pathogenic isolates harboured one to four virulent genes either single or in
combination. The number of l’. enteroco/itica isolates identified in all these three methods was almost
same, Molecular method identified more number of isolates than that of commercial kit method which
may be due to the accuracy of PCR method. The results found clearly show that the molecular and
conventional biochemical methods are equally reliable for detection of contamination of milk with
Yersinia sp.
Description
TNV_EJDS_2010_38(2)1
Keywords
Veterinary Science