RAPD-PCR CHARACTERIZATION OF CAMPYLOBACT ER JEJUNI ISOLATES OBTAINED FROM RAW MILK SAMPLES IN CHENNAI
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Date
2009
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Abstract
A study was conducted to investigate strain variations among 42 C. jejuni iso—lates obtained
from raw milk by Randomly amplified polymorphic DNA (RAPD) method two randomly designed
primer of 10-mers .RAPD profiles were generated with one primer(7718) and the other one(129O) was
unable to do so.The resultant finger prints comprised between 7 and 16 bands for Cjejuni isolates with
sizes between 70 bp and 2400 bp able to amplify C. jejuni DNA .The 13 distinct banding profiles
generated by RAPD were analyzed using computer software and the prepared dendrogram has
grouped the isolates in to four major clusters. The RAPD assay has been proved to have excellent
discrimination ability due to the fact that the entire genome is the target in genotyping . It can be
considered the most advantageous assay in terms of discrimination ability, cost and ease.
Description
TNV_EJDS_2009_37(2)3
Keywords
Veterinary Science