Serosurveillance of Bovine Brucellosis Using RBPT and c-ELISA and Comparative Evaluation of Test Performance
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
The present study was conducted to evaluate the presence of Brucella infection amongst
the randomly selected cattle and buffalo’s population in and around Chennai. The Rose
Bengal Plate Test (RBPT) and Bru Alert® Brucella Antibody c-ELISA was employed to
screen 355 sera samples (295 from cattle and 60 from buffalo) for the presence of Brucella
antibody. The overall positivity of 8.73 % (31/355), 10.14 % (36/355) in bovine
population, within this 9.15 % (27/295), 11.19 % (33/295) among white cattle and 6.66%
(4/60), 5 % (3/60) among buffaloes by RBPT and c-ELSA respectively. Comparatively
high seropositivity was found in >5 years age groups, females and exotic cross breed
animals. The agreement between the two test was excellent (Kappa=0.8847) and also, chisquare
test indicated an evidence of strong (P<0.01) association between the tests. Based
on this study, c-ELISA has been found to be a gold standard test for detecting Brucella
antibodies having more sensitivity and specificity. So on, it is recommended that RBPT
could be successfully used for initial screening of brucellosis in bovines and c-ELISA
should be used as confirmatory test to eliminate false positive results amongst positive
sera.
Description
TNV_IJCMAS_2019_8(8)559-568
Keywords
Veterinary Science