The aim of this research was to test the CHD (Chromo Helicase DNA-binding gene) gene as molecular marker for sexing dimorphic and monomorphic birds because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. Sex determination was attempted in 74 samples of 10 ratite and one non ratite species. Genomic DNA was isolated from blood samples of dimorphic birds and feather samples of monomorphic birds. The ampli cation of the CHD gene was performed with 2550F/2718R sets of universal primers and it was successful in non-ratite species. w1 and k7 primers were suitable for ratite type of birds.