Molecular Marker Based Sex Identification in Ratite and Non-Ratite type of Birds: A Comparative Study
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Date
2017-05
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Association
Abstract
The aim of this research was to test the CHD
(Chromo Helicase DNA-binding gene) gene as
molecular marker for sexing dimorphic and
monomorphic birds because of its high degree of
conservation and different lengths in Z and W
chromosomes due to different intron sizes. Sex
determination was attempted in 74 samples of
10 ratite and one non ratite species. Genomic
DNA was isolated from blood samples of dimorphic
birds and feather samples of monomorphic
birds. The ampliļ cation of the CHD gene was
performed with 2550F/2718R sets of universal
primers and it was successful in non-ratite
species. w1 and k7 primers were suitable for
ratite type of birds.
Description
TNV_IVJ_2017_94(5)11-13
Keywords
Veterinary Science