Expression of Biomarkers in Canine Mammary Tumours
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
Biomarkers identifiable in tumours have potential use in early diagnosis, staging,
prognosis and assessment of response to therapy. This paper describes the
immunohistochemical expression of a few reliable biomarkers in post surgical canine
mammary gland tumour (CMGT). Immunohistochemical expression of biomarkers in
canine mammary tumours was carried out in forty three mammectomised tissue samples.
The intensity and distribution of immunostaining of the biomarkers in histological types of
CMGT was studied. PCNA showed weak nuclear staining in benign tumour and strong
nuclear staining in malignant tumours. Bcl 2 showed weak cytoplasmic staining in benign
tumours and moderate cytoplasmic staining in 10-20% of epithelial cells in malignant
tumours. Cytokeratin 8/18 expression was strong in >50% of epithelial cells in benign and
malignant tumours. Vimentin expression was specific with strong cytoplasmic staining in
the stromal fibrous tissue, stromal fusiform cells, myoepithelial cells in tubules, spindle
cells, chondroid tissue and lipocytes. CEA expression was negative in benign tumours.
Malignant tumours expressed moderate membranous to cytoplasmic staining in 10-20% of
luminal epithelial cells. MUC 1 expression was weak membranous staining in <10% of
apical portion of luminal epithelial cells in benign tumours. Overexpression of MUC 1 was
observed as moderate membranous and cytoplasmic staining in 10-20% of luminal
epithelial cells in malignant tumours. HER-2 expression was weak membrane staining in
<10% of luminal epithelial cells in benign tumours. Moderate membranous and
cytoplasmic staining was observed in 10-20% of luminal epithelial cells in malignant
tumours. COX 2 enzyme expression was negative in benign tumours. Moderate
cytoplasmic staining in 10-20% of luminal epithelial cells, myoepithelial cells, stromal
fusiform cells and stromal inflammatory cells was observed in malignancy. Hence the
expression of biomarkers in tissues is of diagnostic and prognostic significance before
initiation of treatment regimen in canine mammary tumours.
Description
TNV_IJCMAS_2019_8(5)1464-1473
Keywords
Veterinary Science