Short tandem repeat (STR) based genetic diversity and relationship ofdomestic sheep breeds with primitive wild Punjab Urial sheep (Ovisvignei punjabiensis)
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
tIn this study, short tandem repeat (STR) genotypes of 466 sheep from eleven domestic breeds located inAsia, Africa, Europe and Latin America were compared with wild Asian Urial sheep and captive Mouflontype sheep to assess genetic diversity and relationship among them. Analysis of sheep breeds at 19short tandem repeat (STR) loci revealed higher diversity in wild Punjab Urial sheep and domestic sheepfrom West Asia followed by Europe and Latin America as compared to South Asia, consistent with thehistory of sheep domestication. The average inter-individual allele sharing distance within breed wasfound to be lowest in South Asian sheep as compared to West Asian, European and Latin American sheeppopulations. The estimated global FSTrevealed 15.9% of total variation attributable to between breeddifferences. Investigation of genetic relationship by phylogeny revealed four distinct clusters; the firstcluster formed by four South Asian sheep breeds, the second cluster formed by Iraqi and Bulgarian sheepbreeds; the third cluster formed by Iranian, Austrian, Burkina Faso and Peruvian sheep breeds and thefourth cluster formed by Punjab Urial and captive mouflon type sheep. Principal components analysisrevealed individuals from Punjab Urial sheep population being distributed close to the cluster of WestAsian and East European sheep (Hamdani-Karakachanska-West Palminska) while being distinct fromthe cluster of four Indian breeds. Analysis of molecular variance (AMOVA) was performed to assess thedistribution of STR variation as a function of both breed membership and geographic origin, the resultsof which revealed the structure based on phylogeny to be evidently stronger than the structure basedon geographic origin. Bayesian clustering of individuals without prior population information revealedthe most appropriate K with nine genetic clusters. In conclusion, the present study showed the PunjabUrial sheep being genetically closer to West Asian and East European sheep breeds as compared to SouthAsian sheep breeds. The microsatellite based nuclear DNA variations, thus confirmed the wild PunjabUrial sheep did not share any recent common ancestry with Asian domestic sheep, as reported earlierbased on mitochondrial sequence variations
Description
TNV_SSR_2017_148(11-21)
Keywords
Veterinary Science