NUCLEOTIDE VARIATIONS IN LEPTIN (LEP) GENE – A COMPARISON BETWEEN THE NILAGIRI, MERINO AND DORSET x NILAGIRI SHEEP
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Date
2019-06
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TANUVAS
Abstract
LEP gene is one of the potential genes that is involved intricately in the
metabolism and growth of animals. The characterization of LEP gene as compared with the
exotic breeds would help us understand the genetic diversity of indigenous breeds. There
were five variations between the indigenous (Nilagiri), exotic (Merino) and one crossbred
(Dorset x Nilagiri) sheep. In Intron 1 at 13774 bp a ‘GTT’ segment which was present in two
copies in the reference sequence was found as a single copy in Nilagiri. In the intronic
regions that were amplified along with Exon 2, the transitions 13893 T>C (SNP-L1) in Intron
1, 14074 A>G (SNP-L2) and 14090 G>A (SNP-L3) in Intron 2 were identified. For SNP-L1,
Nilagiri and Dorset x Nilagiri had only CC genotypes while Merino had both T and C alleles.
The SNP-L3 was found in Merino and Dorset x Nilagiri breeds while Nilagiri breed did not
vary from the reference sequence. Analysis of the sequenced regions from the amplified
region of Exon 3 revealed one variation 16973 G>A (SNP-L4) which was fixed with A allele
in all the breeds studied here as compared to G in the reference sequence.
Description
TNV_IJSET_2019_8(3)591-594
Keywords
Veterinary Science