Studies were conducted to know the effect of fertilizer, spacing and physiological maturity of seed on seed yield and quality in marigold at Agricultural collage farm, university of Agricultural Science, Dharwad during Kharif 1999. The treatments included are three levels of fertilizes (F1-225:60:60, F2=270:72:72, and F3=315:84:84 NPK kg/ha) and five plants spacings (S1=30*30, S2=45*30, S3=45*45,S4=60*30, and S5=60*45 cm) and experiment was laid out in split plot design with three replications. For the purpose of knowing physiological maturity of seeds heads (capitula) were harvested at seven stages from 13 to 43 days after flower opening (DAF) with an interval of five days. The results indicated that significantly higher yield of good quality seed (579.7 kg/ha) was noticed with the application of 270:72:72 kg NPK per hectare with 60x30 cm spacing (55555 plants/ha) and also it recorded the highest net returns per hectare (Rs.l,17,863). Further increase in fertilizer level to 315:84:84 kg per hectare at the same spacing level did not cause any appreciable effect on seed yield and quality.Among the seven stages of harvesting, seeds harvested at 28 days after fiower opening recorded maximum 1000 seed weight (3.19 gt germination (83%), seedling length (11.92 cm) and vigour index (988) as' compared to the seeds obtained from either delayed or early harvested heads.Based on the results it could be concluded that fertilizer level of 270.72:72 kg per hectare with the spacing of 60x30 cm appeared to be the optimum levels for quality seed production of marigold and the seed crop should be harvested at 28 days after flower opening.