Assessment Of Protein, Lysine And Starch Contents In Grains And Biochemical Markers Associated With Drought Tolerance In Sorghum Genotypes
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Date
2010
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Mahatma Phule Krishi Vidyapeeth, Rahuri.
Abstract
The present investigation was undertaken to evaluate the levels of crude protein, lysine and starch from the sorghum grains of seven each of drought tolerant and susceptible genotypes. In addition, the levels of proline, glycine betaine, the activity of P5CS and soluble proteins were determined from the leaves of these genotypes by imposing stress created by polyethylene glycol-6000. Among the drought tolerant genotypes under study, the highest crude protein content of 9.63% was observed in the grains of RSV-962, whereas among the drought susceptible ones, the highest of 9.24% was observed in CSV-216. Amongst the drought tolerant genotypes, the highest lysine content of 2.19 g 16 g-1 N was observed in the grains of RSV-458 and among the susceptible ones, the highest of 2.17 g 16g-1 N was observed in SPV-504. As regards the starch content, a drought tolerant genotype SPV-1546 recorded the highest of 77.40 per cent in the grains, whereas among the drought susceptible ones, RSLG-1119 recorded the highest of 76.50 per cent. The seedlings of 2-weeks-old sorghum genotypes were evaluated for the accumulation of free proline, glycine betaine and P5CS activity under control and PEG induced stress of -0.5 MPa. On imposition of stress the mean proline accumulation increased by 7-folds in drought tolerant genotypes as compared to a 3-folds increase in drought susceptible ones. The highest proline content of 544.4 μmol g-1 fr.wt. was recorded in a drought tolerant genotype, RSLG-262. The activity of the rate-limiting enzyme, P5CS in leaf material of drought tolerant genotypes demonstrated correspondingly higher activity than the susceptible ones under PEG induced stress. The mean fold increase of 2.44 in P5CS activity of leaf materials was observed in drought tolerant genotypes, whereas there was 1.44-fold in drought susceptible ones. The maximum fold increase of 3.42 in P5CS activity was observed in a drought tolerant genotype, Sel-3. As regards the accumulation of glycine betaine, the mean fold increase of 3.71 was observed in drought tolerant genotypes as compared to 1.57-fold in leaves of a drought susceptible ones. RSV-458 and among the susceptible ones, the highest of 2.17 g 16g-1 N was observed in SPV-504. As regards the starch content, a drought tolerant genotype SPV-1546 recorded the highest of 77.40 per cent in the grains, whereas among the drought susceptible ones, RSLG-1119 recorded the highest of 76.50 per cent.
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