CHARACTERIZATION AND EVALUATION OF BETTER PERFORMING NATIVE ISOLATES OF PHOSPHORUS SOLUBILIZING BACTERIA AND AZOTOBACTER
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Date
2019
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
The present study was conducted in laboratory and green house under controlled conditions at Dept. of Agril. Microbiology, College of Agriculture,IGKV, Raipur, C.G. during the year 2018-19 to characterize and screen different native isolates of PSB and Azotobacterand the better performing isolates were tested for their effect on growth of wheat when they applied alone or in combination. Best performing Azotobacter and PSB isolates were taken under study. Fourteen microbial isolates were biochemically characterized and screened in in-vitro for their plant growth promoting properties. Among 14 tested isolates Azoto-B-44, Azoto-146, Azoto-B-126, PSB-S-88, PSB-H-27, PSB-S-170, PSB-S-71, PSB-H-5,PSB-S-162 were shown positive results for Triple sugar iron agar test and Citrate test. PSB-172 and PSB-S-64 were shown positive for Methyl red test and PSB-S-88, PSB-S-71, PSB-H-5, PSB-S-165 and PSB-S-162 were shown positive for Gelatin liquefaction test. Rest of all isolates were found negative towards above tests. All the isolates were tested for their antibiotic susceptibility property. Seven isolates were found susceptible for Tetracycline (30mcg) and three isolates were found susceptible for streptomycin (10mcg). In N-fixation study of Azotobacter isolates Azoto-B-126 found highest N-fixer. It fixed 3.25mg N/gm of sucrose. Azoto-123, Azoto-146 and Azoto-B-126 found significantly superiorfor N-fixing capacity over Azoto-B-44. Ten isolates of PSB were screened for theirP-solubilizing capacity. PSB-H-5 was found highest P-solubilizer (794.51 µg P/ml), however local isolate PSB-S-71 found at par with PSB-H-5 having solubilization of 768.08 µg P/ml. All the PSB isolates were tested for their solubilizing efficiency by the formation of solubilization zone. PSB-H-27 were found highest solubilization efficiency with solubilization zone of 14 mm diameter, however it was found at par with PSB-S-162, PSB-S-165, PSB-S-71, PSB-H-5 and PSB-S-170.
Out of fourteen isolates, 4 Azotobacter (Azoto-123, Azoto-B-44, Azoto-B-126 and Azoto-146) and 2 PSB isolates (PSB-170 and PSB-S-71) were taken on the basis of their performance in a greenhouse study to find out their effect on the performance of wheat crop. The pot experiment was laid out in CRD with three replication and fifteen treatments involving selected better performing Azotobacter and PSB isolates which were applied alone and in dual combinations with recommended NPK dose. The treatments were T1 (Azoto-123), T2 (Azoto-B-44), T3 (Azoto-B-126), T4 (Azoto-146), T5 (PSB-S-71), T6 (PSB-170), T7 (Azoto-123 + PSB-S-71), T8 (Azoto-123 + PSB-170), T9 (Azoto-B-44 + PSB-S-71), T10 (Azoto-B-44 + PSB-170), T11 (Azoto-B-126 + PSB-S-71),T12 (Azoto-B-126 + PSB-170), T13 (Azoto-146 + PSB-S-71), T14 (Azoto-146 + PSB-170) and T15 (Uninoculated control).
Among different treatments dual inoculation of Azoto-B-126 and PSB-S-71 (T11) was found best for different growth parameters of wheat crop followed by T14 (Azoto-146 and PSB-170) at 60 DAS. The highest plant height (72.7 cm) was found in treatment T11 (Azoto-B-126+PSB-S-71) followed bytreatment T14 (Azoto-146+PSB-170: 70.6 cm). Maximum fresh weight of plant(7.44 gm/plant) was associated with treatment T11 which was at par with treatment T14 (Azoto-146+PSB-170: 7.33 gm/plant), T12 (Azoto-B-126+PSB-170: 7.05 gm/plant) and T6 (PSB-170: 7.11 gm/plant).Similarly highest plant dry weight(2.52 gm/plant) was associated with T11 (Azoto-B-126+PSB-S-71) which was at par with treatment T14 (Azoto-146+PSB-170: 2.48 gm/plant) and T6 (PSB-170: 2.47 gm/plant). It was concluded from the study that dual inoculation of Azoto-B-126 and PSB-S-71 was found best for increasing biomass yield of wheat crop by increasing nitrogen and phosphorus availability to the crop.
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