EVALUATION AND UTILIZATION OF SSR MARKERS FOR GENETIC DIVERSITY CHARACTERIZATION IN SEABUCKTHORN.
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Date
2019-07
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Publisher
CSKHPKV, Palampur
Abstract
Seabucktthorn (Hiippophae rhamnoiides,, diiplloiid 2n=24) iis a diioceous,, wiind
polllliinatted,, tthorny shrub bellongs tto famiilly Ellaeagnaceae.. In Indiia,, iitt grows natturalllly
iin hiigh allttiittude and colld-ariid regiions of Ladakh,, Siikkiim,, Jammu & Kashmiir,,
Hiimachall Pradesh,, and Uttttrakhand.. Itt iis economiicalllly and ecollogiicalllly iimporttantt
mediiciinall pllantt due tto iitts hiigh iimmense mediiciinall properttiies butt tthere iis
unavaiillabiilliitty of superiior variietty// sellecttiion avaiillablle for llarge scalle commerciiall
cullttiivattiion iin Indiia.. Furtthermore,, valliidatted miicrosattelllliitte marker resources are allso
lliimiitted iin tthiis pllantt speciies.. Therefore,, a ttottall of 882 novell SSR markers were miined
from iin-house H.. rhamnoiides ttranscriipttome datta.. Among allll,, abundance of ttrii-repeatts
(49%) follllowed by dii-repeatts (46%) was observed.. Furtther,, evalluattiion and uttiilliizattiion
of pollymorphiic SSR makers for genettiic diiversiitty assessmentt of 245 accessiions of
Seabucktthorn (H.. rhamnoiides) dettectted hiigh average allllelle frequency (5..45 allllelles//
priimer) and hiigh hetterozygosiitty (Ho:: 0..77;; He:: 0..75).. Neiighbor-Joiiniing dendrogram,,
priinciipall coordiinatte anallysiis (PCoA) and Bayesiian modell based popullattiion sttructture
anallysiis grouped allll tthe 245 iindiiviidualls iintto ttwo majjor groups wiitth hiigh wiitthiin
popullattiion gene fllow suggesttiing tthatt tthere are ttwo genettiic poolls operattiing iin tthe
natturall seabucktthorn popullattiions of ttrans--Hiimallayan Leh and Keyllong regiions of
Indiia.. The resulltts of tthiis sttudy wiillll be usefull for iidenttiifiicattiion and sellecttiion of
pottenttiiall genottypes tto expediitte tthe conservattiion and genettiic iimprovementt programme
iin seabucktthorn.. Genome-wiide miicrosattelllliitte marker resource creatted duriing tthiis
sttudy can be valliidatted and uttiilliized for futturiisttiic evolluttiionary and variious genottypiic
sttudiies iin seabucktthorn.
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