An investigation on Clonostachys rosea (Ascomycota: Hypocreales) as an entomopathogenic fungus against mango hopper
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Date
2019-07
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
A study regarding collection, isolation, identification, and pathogenicity test was conducted for the local isolate having standards and potential of being entomopathogenic fungi. This isolate was found and studied in Pantnagar, Udhamsingh Nagar, Uttarakhand; during 2017-19. All the above mentioned heads were studied meticulously and the results told that the local isolate is Clonostachys rosea (Link: Fries) (Ascomycota:
Hypocreales) formerly known as Gliocladium roseum Bainier; teleomorph: Bionectria ochroleuca (Schweinitz); with an effective similarity of 98.80% and 98.63% to KY378958.1 and MH869057.1. Results on standardization of the media deciphered that the best option for mass multiplication of Clonostachys rosea is Oatmeal Agar media (OMA) and Oatmeal Broth (OMB) at a temperature range of 20oC to 25oC when
considering mean radial growth, mean conidia/bit, and mean germination percentage. In the bioassay evaluation of the local isolate against Mango Hoppers (Amritodus atkinsoni), Okra Jassids (Amrasca sp.), Silver Oak Termites (Odontotermes obesis), and Greater Wax Moth larvae (Galleria mellonella); it was found that the highest mean mortality was obtained for Mango Hoppers (96.67%) at a concentration of 3x108
conidia/ml and for Okra Jassids, the highest mean mortality obtained was 80% at a concentration of 3x108 conidia/ml. No death was espied for Silver Oak Termite and Greater Wax Moth larvae. Thus, in culmination it can be inferred that the isolated fungus is a novoentomopathogenic
fungi which has potency to be included in IPM module and there are still many entomopathogenic fungi undiscovered from nature. Much intricate and more such studies are required in Insect Pathology.
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