HOST – PLANT RESISTANCE, MOLECULAR CHARACTERIZATION AND MANAGEMENT OF MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) IN MUNGBEAN
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Date
2018-08-20
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University of Agricultural Sciences, Bengaluru
Abstract
Mungbean Yellow Mosaic Virus (MYMV) is a most destructive and major
limiting factor for mungbean cultivation. Among fourteen genotypes (AVRDC lines)
evaluated for MYMV resistance during summer 2017, Kharif 2017 and summer 2018,
genotypes viz., MYB-6, 7, 8, 9 and 12 showed resistant reaction with low per cent disease
index (PDI) and low cumulative disease progress (AUDPC). Further, the resistant
genotypes were screened and confirmed for MYMV resistance by vector transmission
through whitefly Bemisia tabaci. PCR confirmation was done by using MYMV coat
protein gene specific primers with an amplicon of ~900bp which was detected in all the
genotypes except MYB-6, 7, 8, 9 and 12. Additionally, the presence of MYMV in
resistant lines was confirmed by Rolling Circle Amplification (RCA)-PCR. The
molecular characterization of CP gene of MYMV showed 99.6 per cent sequence identity
with Mungbean Yellow Mosaic Virus Vigna segment A (AJ132575.1). Yellow mosaic
virus disease in mungbean was effectively managed by seed treatment with imidacloprid
600 FS @ 5g kg-1 of seeds + spraying of red sea plant extract LBD-12 (15 DAS) @ 3.0ml
L-1 + SEVI [(Synthetic Nucleotide Analog) (30 DAS)] @ 2.0ml L-1 and thiomethoxam 25
WG (45 DAS) 0.5g L-1 (T6) with low PDI (33.89 %), low AUDPC value (1412.40) and
high yield (5.15 q acre-1) compared to control (PDI=55.87 %, AUDPC=2056.13,
yield=3.97 q acre-1). Interestingly, the same treatment showed increased activity of
enzymes like peroxidase, polyphenol peroxidase and phenylalanine ammonia lyase and
defence inducing compounds like total phenols and total sugars.
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