Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810111723
Authors: NAGARAJ
Advisor: Nagaraju, N.
Title: HOST – PLANT RESISTANCE, MOLECULAR CHARACTERIZATION AND MANAGEMENT OF MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) IN MUNGBEAN
Publisher: University of Agricultural Sciences, Bengaluru
Language: en
Type: Thesis
Pages: 127
Agrotags: null
Abstract: Mungbean Yellow Mosaic Virus (MYMV) is a most destructive and major limiting factor for mungbean cultivation. Among fourteen genotypes (AVRDC lines) evaluated for MYMV resistance during summer 2017, Kharif 2017 and summer 2018, genotypes viz., MYB-6, 7, 8, 9 and 12 showed resistant reaction with low per cent disease index (PDI) and low cumulative disease progress (AUDPC). Further, the resistant genotypes were screened and confirmed for MYMV resistance by vector transmission through whitefly Bemisia tabaci. PCR confirmation was done by using MYMV coat protein gene specific primers with an amplicon of ~900bp which was detected in all the genotypes except MYB-6, 7, 8, 9 and 12. Additionally, the presence of MYMV in resistant lines was confirmed by Rolling Circle Amplification (RCA)-PCR. The molecular characterization of CP gene of MYMV showed 99.6 per cent sequence identity with Mungbean Yellow Mosaic Virus Vigna segment A (AJ132575.1). Yellow mosaic virus disease in mungbean was effectively managed by seed treatment with imidacloprid 600 FS @ 5g kg-1 of seeds + spraying of red sea plant extract LBD-12 (15 DAS) @ 3.0ml L-1 + SEVI [(Synthetic Nucleotide Analog) (30 DAS)] @ 2.0ml L-1 and thiomethoxam 25 WG (45 DAS) 0.5g L-1 (T6) with low PDI (33.89 %), low AUDPC value (1412.40) and high yield (5.15 q acre-1) compared to control (PDI=55.87 %, AUDPC=2056.13, yield=3.97 q acre-1). Interestingly, the same treatment showed increased activity of enzymes like peroxidase, polyphenol peroxidase and phenylalanine ammonia lyase and defence inducing compounds like total phenols and total sugars.
Subject: Plant Pathology
Theme: HOST – PLANT RESISTANCE, MOLECULAR CHARACTERIZATION AND MANAGEMENT OF MUNGBEAN
These Type: M.Sc
Issue Date: 2018-08-20
Appears in Collections:Theses

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