The experiment was conducted in three parts, in first part; in-vitro trial was conducted for production of organic zinc by using different strains of yeast. In second part, a biological trial was conducted on broilers using different levels of inorganic zinc to know the best level based on the performance which was used in the next trial. In third part, organic zinc trial was conducted on broilers along with selected level of inorganic zinc. In-vitro- Production of zinc enriched yeast was carried out by utilizing five different strains which were used to know the suitable yeast for biomass production and level of zinc incorporation under different concentrations of zinc i.e 0, 30, 60, 90, 120 ppm from three different sources of zinc i.e ZnSO4, ZnCl3 and ZnNO3. The best strain, zinc source and concentration were selected based on the highest increment of zinc in biomass. Based on the results, yeast strain SC 050 with the concentration of 120 ppm and zinc source as zinc sulphate were selected for production of zinc enriched yeast. Trial I-A biological trial was conducted by supplementing different levels of inorganic zinc in the form of ZnSO4 for selecting the best level of inorganic zinc based on the performance of broilers. The experiment was carried out on 300 birds which were distributed in to 6 treatment groups in such a way that each treatment group had 10 replicates with 5 birds per replicate. Inorganic zinc was supplemented at 0, 20, 40, 60, 80 and 100 ppm levels. The weekly body weights, gain, feed intake, FCR and livability were studied. Based on the results, 100 ppm level of inorganic zinc had shown highest body weights (1830.3) and weight gain (1778.4) during overall period. The cumulative feed intake was better (P<0.05) in 100 ppm (3037.5) supplemental groups and the lowest feed intake was recorded in 0 ppm (2954.9). Cumulative FCR was found to be better (P<0.05) at 100 ppm (1.707) when compared with all other supplemented groups. Based on the performance 100 ppm organic zinc was selected for the next trial. Trial II- Growth trial was conducted by using different levels of organic zinc (zinc enriched yeast) i.e. 0, 20, 40, 60, 80, 100 and one level of inorganic zinc INO 100 ppm which was selected from trial I. The experiment was carried out on 350 broiler chicks distributed in to 7 treatment groups in such a way that each treatment group had 10 replicates with 5 birds per replicate. The highest (P<0.05) weight gain was observed in 60 (2308.02) and 100 ppm (2309.42) organic supplemented groups and lowest body weight gain were recorded in 0 ppm (2204.18). No significant (P>0.05) difference was observed for cumulative feed intake among all the treatment groups. Cumulative FCR showed a significant (P<0.05) improvement at 60 (1.636) and 100 ppm (1.631) and poor FCR was recorded in INO 100 ppm (1.683) group. Dressing percentage did not show significant (P>0.05) difference when zinc was supplemented at different levels. Immune response pertaining to humoral and cell-mediated immunity showed improvement with the supplementation of organic zinc and the humoral response was found to be higher at 80 and 100 ppm and cell-mediated immune response at 100 ppm of organic zinc supplementation.When diets were supplemented with 100 ppm organic zinc improvement was observed in Glutathione peroxidise, Glutathione reductase, SOD along with lowest level of lipid peroxidation. No difference was observed in livability percentage for all the treatment groups. Digestibility of drymatter, crude protein retention and zinc retention showed better at 100 ppm of organic zinc. Significant (P<0.05) difference was observed at 100 ppm of organic zinc supplementation in the total protein. Lowest cholesterol value was recorded in 100 ppm (144.88) of organic and highest cholesterol was observed in 0 ppm (150.07) group. The deposition of zinc in bone, breast and liver was found to be higher in 100 ppm organic zinc and lowest deposition was observed in 0 ppm group. No significant difference was observed between 60 ppm organic zinc and INO 100 ppm. Significant (P<0.05) difference was observed for 80 and 100 ppm organic for serum zinc level when compared to other zinc supplemented groups. Based on the overall results of the experiments, it can be concluded that zinc enrichment depends on type of strain, zinc source and concentration of zinc in media. Highest biomass yield was observed at 30 ppm and increment at 120 ppm. In the trial with inorganic zinc, 100 ppm level showed better performance as compared to other levels. When organic zinc was compared with INO 100 ppm, organic zinc was found to be significantly better in terms of performance, immune response and antioxidant status. But 60 ppm level was comparable with 100 ppm inorganic zinc with better results. More zinc excretion was observed in 100 ppm inorganic zinc as compared to organic zinc supplementation.