EXTREME HOT AMBIENT TEMPERATURE VIS-À-VIS ANTIOXIDANT POTENTIAL OF LIVER AND PLASMA IRON STATUS IN MARWARI SHEEP FROM ARID TRACTS
Loading...
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Rajasthan University of Veterinary and Animal Sciences, Bikaner
Abstract
The present investigation was carried out to appraise the extreme hot ambient temperature vis-à-vis antioxidant potential of liver and plasma iron status in Marwari sheep from arid tracts. To achieve the objectives of the investigation, blood and liver samples were collected from Marwari male and female sheep of 4 months to 10 months of age groups during congenial (months of October and November) and extreme hot (May, June and July) ambient temperature periods (ATPs)from private slaughter houses. The male and female Marwari sheep were categorized as 4-6 months; 6-8 months and 8-10 months of age groups in each ambient temperature period (ATP). To accomplish the objectives, parameters were quantified in the liver and plasma (where ever applicable) during congenial and extreme hot ATPs. The parameters of antioxidant potential of liver included superoxide dismutase (SOD); catalase (CAT); glutathione reductase (GR); malondialdehyde (MDA); peroxidase (PO); vitamin E; vitamin C and glutathione and parameters of plasma iron status incorporated plasma iron and total iron binding capacity. For each parameter, control mean value was obtained during congenial ATP and the mean value obtained during extreme hot ATP was compared from control value of respective parameter. Effect of gender and age group was also contemplated. Appraisal of the extreme hot ambient temperature vis-à-vis antioxidant potential of liver and plasma iron status was carried out on the basis of per cent changes in parameters during extreme hot ATP. Highly significant (p≤0.01) interactions of gender X age group; ATPs X gender; ATPs X age groups; ATPs X gender X age groups for markers of liver antioxidant potential and plasma iron status divulged the supremacy of influences in each group. Extreme hot ATP caused a significant (p≤0.05) increase in the mean values of liver SOD, CAT, GR, MDA and PO and a significant
ii
(p≤0.05) decrease in the mean values of vitamin E, vitamin C and glutathione; and plasma iron and total iron binding capacity as compared to respective congenial ATP mean value. Significant (p≤0.05) difference in the mean values between male and female Marwari sheep for each parameter denoted that gender impact was commanding. For each parameter, mean value obtained from male Marwari sheep was found to be elevated significantly (p≤0.05) as compared to respective mean value taken from female Marwari sheep. Maximum per cent change in the mean value of each parameter due to extreme hot ATP was observed in the female Marwari sheep. Precedent of alterations in the values of parameters of antioxidant potential in the liver during extreme hot ATP reflected the existence of oxidative stress of a comparatively greater degree in female animals. The upshot of the study indicated that male Marwari sheep had better antioxidant potential of liver as compared to female animals. Per cent change in the values indicated the ability of the animals to maneuver the mechanisms during the extreme hot ATP. It can be stated that though female animals had lowered levels of each parameter of antioxidant potential, but the ability to fight the oxidative stress was greater which was reflected in terms of changes in the levels. Similarly, iron status also showed significant alterations in the values. Lowering of the values was observed during extreme hot ATP. Per cent lowering was greater in female animals than in male animals. Mean levels of iron status were comparatively higher in the male animals than the female animals. On the basis of maximum value of per cent change, the sensitivity level of a parameter during extreme hot ATP was also determined. Since liver malondialdehyde revealed maximum per cent increase, it was substantiated to be as most sensitive parameter of antioxidant potential of the liver. It can be projected that female Marwari sheep and Marwari sheep of 4-6 months of age were observed to be most affected with regards to antioxidant potential of the liver due to extreme hot ATP. It can be deduced that extreme hot ambient temperature period affected the antioxidant potential and iron status of male and female Marwari sheep of all the age groups investigated. It can be suggested that animals of arid tracts must be shielded from long exposure to extreme hot ambient temperature. Regular supplementation of suitable antioxidants is recommended to save the animals from the peril of extreme ambient temperatures by enhancing the antioxidant potential.
Description
Keywords
null