Micropropagation in selected varieties of Anthurium andreanum Lind.
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Date
1996
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Department of Horticulture, College of Agriculture, Vellayani
Abstract
Studies were conducted to optimise the in vitro propagation techniques via somatic organogenesis in Anthuruim andreanum va~ieties (Dragon's Tongue, Flaking, Pompon Red
Honeymoon Red and Nitta) during 1994-1995 at the Department
of Horticulture, College of Agriculture, Vellayani.
All the five varieties responded to the callusing
treatments in varying degrees. Regeneration was obtained only
in the variety Dragon's Tongue and this variety was
subjected to different treatments for refinement of callusing and shoot proliferation. The protocol for in vitro
propagation of the variety Dragon's Tongue could be
standardised.
l\mong the different explants tried only leaf
explants were found responsive for callusing. Callus was
best initiated (50.0 % ) within 60 days when leaf explants
were cultured in darkness on modified Murashige and Skoog
basal medium (NH4N03 200 mg/l) supplemented with 2,4-D 0.5
mg/l, BA 0.5 mg/l, sucrose 30.0 g/l and agar 6.0 g/l.
The callus cultures were subcultured in the same
medium for two months for callus multiplication.
Regeneration was obtained within one month on
Murashige and Skoog basal medium supplemented with BA 0.5
mg/l, IAA 2.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l. Light
was essential for regeneration.
The shoots) was supplemented hydrolysate maximum rate of shoot proliferation (13.49
observed on Murashige and Skoog basal medium
with kinetin 1.5 mg/l, IAA 3.0 mg/l, casein
150.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l
after a period of six weeks.
Improvement in growth of shoots was obtained by
culturing in Murashige and Skoog basal medium supplemented
with activated charcoal (1.0 g/l) and further subculturing
to Murashige and Skoog basal medium supplemented with
kinetin 0.5 mg/l and IAA 16.0 mg/l.
A separate rooting phase was not necessary since
satisfactory rooting was obtained in the shoot proliferation
medium itself.
Rooted plantlets gave a survival rate of 60.0 per
cent on planting out.
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PG
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Citation
171420