Effect of zno and fe2o3 nanoparticles as released from an alternate nutrient sources in in vitro culture media on various biomolecules Picrorhiza kurroa Royle ex Benth
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Date
2018-12
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Genus Picrorhiza and their different species from varied habitat have been recognized and proven for various ailments, significant contribution from India has a potential due to the enormous biodiversity with special reference to native species collected from either from cultivated at various micro and macro environment or otherwise. Picrorhiza kurroa Royle ex Benth is one of the precious herbs of genus Picrorhiza. Several iridoid glycosides are present in this plant which includes their derivatives having various pharmacophore, are being subjected for their utilization in several modern and traditional medicines systems to cure number of ailments since ancient time. The plant is endemic and has been vastly explored. At present it is well recognized for hepatoprotection. It is essential to apply biotechnological approaches to enhance the production of various pharacophore either by extracts and their purifications or semi synthesis from in vitro as well as in vivo. Application of nanoscience related technologies from the bio mass through various culturing practices in various environments, have provided versatility with the secondary metabolites. During the present research work, various attempts have been made to optimized protocols for in vitro regeneration of Picrorhiza kurroa along with the effect of ZnO and Fe2O3 nanoparticles as an alternate nutrient source to recognize some facts about the synthesis of secondary metabolites, antioxidative enzymes (SOD, CAT, POX, APOX) and expression of rate limiting genes ((CAM, HMGR, DXS, EPSPS, GDPS, PAL)) involved in the synthesis of Picroside II. Nanoparticles study results indicated that it might be possible that IPP for biogenesis of different iridoid glycoside and their derivatives under application of their precursor the mevalonate, non mevalonate and phenyl-propanoid pathways might enhance the Picroside II. ZnO and Fe2O3 nanoparticles have been proved better method for eliciting their effect in order to enhance picrosides production.
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