Studies on Differential Response of French Bean Genotypes Against Aluminium toxicity in North East Region.

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Date
2018-12-02
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College of Horticulture and Forestry, Central Agricultural University, Pasighat
Abstract
The experiment was conducted in naturally ventilated polyhouse at Vegetable Research Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, East Siang, Arunachal Pradesh, India in 15 genotypes of French Bean (Phaseolus vulgaris L.). The experiment was laid out in Completely Randomized Design (CRD) in factorial concept with two factors, the genotype as first factor with 15 genotypes and aluminium as second factor with four levels. Morpho-physiological parameters, quality parameters and soil parameters were recorded after 15 days and 30 days of aluminium application whereas yield parameters were taken at harvesting stage. The genotypes include G1 (Lakshmi), G2 (Swarna Lata), G3 (RCMFB-1), G4 (Mizoram), G5 (Meghalaya), G6 (Tripura), G7 (Pasighat, Arunachal Pradesh), G8 (Mao, Manipur), G9 (Mao, Manipur), G10 (Choinu village, Manipur), G11 (Chandel District, Manipur), G12 (Mayangkhang village, Manipur), G13 (Mayangkhang village, Manipur), G14 (Senapati, Manipur) and G15 (Senapati, Manipur). G15 proved to be most superior for plant height among all the genotypes. For other growth parameters like biomass and dry matter, G1, G10 and G15 were found to be superior and for root length genotypes G1, G2, G14 and G15 were found tolerant to aluminium toxicity. In soil parameters, pH and available P decreased with Al treatment, while extractable Al increased significantly with respect to control. All the yield parameters were significantly reduced due to Al treatment. However, among different genotypes G4, G11, G8, G3 and G15 were found to be superior for yield. Pod length and average pod weight was found superior in G3 and G7. G4 was found superior for number of seed per pod. Among all the genotypes the highest chlorophyll a, chlorophyll b and total chlorophyll content was found in G13. Highest total protein content was found in genotype G1 and highest relative water content was found in G4. However there was significant increase in carotenoid content and highest content was observed in G12. The ROS activity as well as lipid peroxidation was found more prominent in the roots as compared to the shoots and with the increase in aluminium concentration there ROS activity and lipid peroxidation also increases in both shoot and root. High level of ROS and lipid peroxidation was recorded at 40 ppm and 60 ppm when compared with the control (0ppm). Highest superoxide anion (O2.-) content was observed in G9 in shoot and G4 in roots. Hydrogen peroxide (H2O2) content was found highest in G13 in shoots and G3 in roots. The highest value of hydroxyl free radical OH. in shoots was recorded at G14 and in roots as G15. G8 in shoots and G4 in roots recorded the highest lipid peroxidation content. The highest CAT activity was observed in G3 at 30 days in shoot and G8 in roots. G13 recorded the maximum superoxide dismutase (SOD) activity in shoot and G15 in roots. The maximum value of guaiacol peroxidise (GPX) in shoot was recorded G10 and G9 in root. Non-enzymatic antioxidants like ascorbic acid content and vitamin E content was significantly influenced by genotypes and aluminium treatment. At 30 days maximum ascorbic acid content was recorded in Genotype G6 in shoots and G3 in roots. The vitamin E content was recorded maximum in G1 in shoots and G13 in roots. The proline content significantly increased in aluminium treated plants as compared to control. Proline accumulation was found maximum in G12.
Description
The experiment was conducted in naturally ventilated polyhouse at Vegetable Research Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, East Siang, Arunachal Pradesh, India in 15 genotypes of French Bean (Phaseolus vulgaris L.). The experiment was laid out in Completely Randomized Design (CRD) in factorial concept with two factors, the genotype as first factor with 15 genotypes and aluminium as second factor with four levels. Morpho-physiological parameters, quality parameters and soil parameters were recorded after 15 days and 30 days of aluminium application whereas yield parameters were taken at harvesting stage. The genotypes include G1 (Lakshmi), G2 (Swarna Lata), G3 (RCMFB-1), G4 (Mizoram), G5 (Meghalaya), G6 (Tripura), G7 (Pasighat, Arunachal Pradesh), G8 (Mao, Manipur), G9 (Mao, Manipur), G10 (Choinu village, Manipur), G11 (Chandel District, Manipur), G12 (Mayangkhang village, Manipur), G13 (Mayangkhang village, Manipur), G14 (Senapati, Manipur) and G15 (Senapati, Manipur).
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