Seed quality assessment in natural and artificially aged seed of fenugreek (Trigonella foenum-graecum L.)

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Date
2019
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CCSHAU
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The present study entitled “Seed quality assessment in natural and artificially aged seed of fenugreek (Trigonella foenum-graecum L.)” was carried out in the Department of Seed Science & Technology at CCS Haryana Agricultural University, Hisar (Haryana). The experiments were laid out in factorial Complete Randomized Design (CRD) and Randomized Block Design (RBD) for laboratory and field parameters, respectively on six genotypes of fenugreek viz., HM-57, HM-103, HM-273, RMT-361, FGK-49, FGK-80. The present investigation was carried out in three different experiments to meet out the objectives of study. In the first experiment, To assess the seed quality during natural ageing at 3 months interval up to 18 months and check physiological and biochemical parameters viz., standard germination, seedling length, seedling dry weight, vigour index-I & II, viability test (Tz %) catalase (CAT) peroxidase (POX), superoxide dismutase (SOD), dehydrogenase enzyme activity parameters were decreased significantly with the advancement of natural ageing of seeds whereas, electrical conductivity of seeds leachates increased with the advancement of time. The field parameters viz., seedling emergence index and seedling establishment were also decreased significantly with the advancement of time whereas, mean emergence time increased with the passage of time. In the second experiment, to study the physiological and biochemical change after accelerated ageing of all six genotypes of fenugreek. Effect of artificial ageing was more as compared to natural ageing to degradation fresh seed. In the third experiment effect of priming on marginal seed of natural and artificial aged seed of all six genotypes of fenugreek. Seed priming treatments viz., T0: Control, T1: hydro-priming followed by dry dressing with thiram @ 0.25%, T2: Hydration with GA3 (50 ppm), T3: Hydration with PEG (6000), T4: Hydration with KNO3 (0.5%), T5: 2% CaCl2 were analyzed to identify the suitable priming treatment. Among various priming treatments „T2‟- hydration with GA3 @ 50 ppm followed by „T1‟-Thiram performed best to enhance all the seed vigour and viability characteristics and to lower down the electrical conductivity of naturally and artificially aged seed of fenugreek. The seed quality improvement through seed priming was noticed more in marginal seed i.e. artificial aged seed. Among the different genotypes, maximum enhancement was observed in FGK-80 followed by FGK-49 whereas, minimum enhancement was observed in genotype HM-57 during the study. In conclusion, the present study revealed that fenugreek seed lose its viability and vigour with the advancement of storage time and seed priming with GA3 @ 50 ppm and thiram can be used as an effective tool to enhance vigour and viability of seed.
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