Tagging gene(s) for resistance to wilt in lentil (Lens culinaris Medikus subsp. culinaris)
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Date
2018
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Abstract
Fusarium wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious
disease of lentil (Lens culinaris Medikus), causing severe yield losses worldwide and
it is a major disease in Central India. The disease is soil borne causing huge losses and
development of wilt resistant varieties is most effective means of controlling this
disease. Highly resistant sources of wilt in lentil have not been reported from the Indian
lentil breeding programme. Ninety three lentil accessions including twelve varieties,
six ICARDA germplasm lines and seventy five advanced breeding lines were evaluated
in field and controlled conditions against wilt. Among which six genotypes IG 69549,
IG70238, IG71487, ILL10916, ILL10921 and ILL10965 were identified as resistant in
wilt sick plot as well as greenhouse condition. The germplasm L9-12 as wilt susceptible
parent and ILL10965 as wilt resistance parent were used to develop mapping
population. The inheritance of wilt-resistance gene in lentil was investigated in F2
mapping population (L9-12 × ILL10965) exhibited 3:1 ratio of resistance and
susceptible plants and F2:3 mapping population plants exhibited 1:2:1 ratio for
resistance, segregating and susceptible reaction respectively. It confirmed that wilt
resistance in lentil is dominant over susceptibility without any maternal effect and
controlled by a single dominant gene. Both wilt resistance gene inheritance and marker
segregation was confirmed by Chi-square test, which fitted well the 3:1 ratio. Three
hundred two SSR markers were surveyed for the parental polymorphism, in which three
SSRs viz. PBALC233, PBALC1409 and PBALC203 were found polymorphic between
parents as well as resistance and susceptible bulks. Linkage analysis showed two SSR
markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and
9.4 cM distance, respectively. Further, PBALC233 was also found present on the same
linkage group at a distance of 10.2 cM from PBALC1409.
Keywords : Fusarium wilt, Gene, Lentil, Mapping Population and Markers
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t-9960
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