Post-Natal Development Of The Prostate Gland In Buffalo (Bubalus Bubalis) : A Histomorphochemical Study
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Date
1998
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The present investigation was undertaken on male buffaloes (n=27) to
study the age related histomorphochemical changes in the prostate gland and to
study experimentally, the epithelial development of the prostate gland in relation
to androgen dependence. For Histomorphochemical study, animals (n=19) were
divided into four groups - neonatal, three month old calves, prepubertal (1 year)
and adult (> 3 years) animals. For experimental study, prepubertal buffalo
calves (1-1'/2 year) (n=8) were divided into control and treatment group.
Treatment group animals were administered 5a-androstane 3a, 17p-diol for 8
weeks.
The prostate gland in buffaloes was composed of two parts - corpus
prostatae and pars disseminate. Corpus prostatae was small, grossly indistinct
and inconspicuous upto 11/2 year of age and inconsistent'y detected grossly in
adult animals. The pars disseminata was scattered in the wall of pelvic urethrabut the relative number and distribution of glandular units and ducts was
markedly variable along its length.
The prostate gland of buffalo was a compound tubulo-acinar and
predominantly serous type of gland. During post -natal development, the
percentage of solid glandular end pieces progressively decreased with
advancing age till in adult animals where all the glandular end pieces were
luminated. Four types of cells viz. principle (serous), mucous, dark narrow and
basal cells were detected in glandular and ductular epithelia. The micrometrical
analysis revealed that the major growth of ducts took place between neonatal
period to 11/2 year of age whereas the growth of tubulo-acinar units occurred
between 11/2 to 3.years of age.
In neonate and three month old calves, the cytoplasm of ductular and
glandular epithelial cells contained very weak PAS reaction. However, in the
prepubertal and adult animals, the cytoplasm of mucous cells as well as
secretory material of ducts and glands showed stronger reactions for both
neutral and acidic mucopolysaccharides. Two type of mucous cells were
detected. The mucous intraepithelial glands detected close to the origin of large
ducts, contained predominently acidic mucopolysaccl-arides and were fully
functional even at neonatal age.
Calcium, iron and copper could not be demonstrated on the tissue
sections of prostate gland in any age groups. In adult animals, the tubulo-acinar
units exhibited stronger reaction for total proteins in thE supranuclear than the
basal parts of cell cytoplasm. Weak reactions were detected for sudanophilic
lipids in all age groups.
The histoenzymic activity of ACPase, ATPase, G -6 -P -D, NADH-D, CYO
and CA showed a progressive increase in the glandular and ductular epithelium
with advancing age. SDH and LDH were high in neonates and adults. AKPase
and AchEase were localised in the subepithelial capillaries and neuronal
components respectively.
I mmunohistochemically detected prostate specific ACPase was
concentrated in the supranuclear and infranuclear cytoplasm of glandular cells in
prepubertal and adult animals.
The results after dihydroandrosterone stimulation were variable among
different animals. Three out of four animals exhibited moderate to intense
stimulation in their central zone, mainly manifested by the changes in the
histology of glandhtlfir units and ducts that resembled to those of adult animals.
o. prizAP._