Post-Natal Development Of The Prostate Gland In Buffalo (Bubalus Bubalis) : A Histomorphochemical Study

Loading...
Thumbnail Image
Date
1998
Journal Title
Journal ISSN
Volume Title
Publisher
Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The present investigation was undertaken on male buffaloes (n=27) to study the age related histomorphochemical changes in the prostate gland and to study experimentally, the epithelial development of the prostate gland in relation to androgen dependence. For Histomorphochemical study, animals (n=19) were divided into four groups - neonatal, three month old calves, prepubertal (1 year) and adult (> 3 years) animals. For experimental study, prepubertal buffalo calves (1-1'/2 year) (n=8) were divided into control and treatment group. Treatment group animals were administered 5a-androstane 3a, 17p-diol for 8 weeks. The prostate gland in buffaloes was composed of two parts - corpus prostatae and pars disseminate. Corpus prostatae was small, grossly indistinct and inconspicuous upto 11/2 year of age and inconsistent'y detected grossly in adult animals. The pars disseminata was scattered in the wall of pelvic urethrabut the relative number and distribution of glandular units and ducts was markedly variable along its length. The prostate gland of buffalo was a compound tubulo-acinar and predominantly serous type of gland. During post -natal development, the percentage of solid glandular end pieces progressively decreased with advancing age till in adult animals where all the glandular end pieces were luminated. Four types of cells viz. principle (serous), mucous, dark narrow and basal cells were detected in glandular and ductular epithelia. The micrometrical analysis revealed that the major growth of ducts took place between neonatal period to 11/2 year of age whereas the growth of tubulo-acinar units occurred between 11/2 to 3.years of age. In neonate and three month old calves, the cytoplasm of ductular and glandular epithelial cells contained very weak PAS reaction. However, in the prepubertal and adult animals, the cytoplasm of mucous cells as well as secretory material of ducts and glands showed stronger reactions for both neutral and acidic mucopolysaccharides. Two type of mucous cells were detected. The mucous intraepithelial glands detected close to the origin of large ducts, contained predominently acidic mucopolysaccl-arides and were fully functional even at neonatal age. Calcium, iron and copper could not be demonstrated on the tissue sections of prostate gland in any age groups. In adult animals, the tubulo-acinar units exhibited stronger reaction for total proteins in thE supranuclear than the basal parts of cell cytoplasm. Weak reactions were detected for sudanophilic lipids in all age groups. The histoenzymic activity of ACPase, ATPase, G -6 -P -D, NADH-D, CYO and CA showed a progressive increase in the glandular and ductular epithelium with advancing age. SDH and LDH were high in neonates and adults. AKPase and AchEase were localised in the subepithelial capillaries and neuronal components respectively. I mmunohistochemically detected prostate specific ACPase was concentrated in the supranuclear and infranuclear cytoplasm of glandular cells in prepubertal and adult animals. The results after dihydroandrosterone stimulation were variable among different animals. Three out of four animals exhibited moderate to intense stimulation in their central zone, mainly manifested by the changes in the histology of glandhtlfir units and ducts that resembled to those of adult animals. o. prizAP._
Description
Keywords
Citation
Collections