STANDARDIZATION OF HARDENING PROTOCOL OF TISSUE CULTURED MULBERRY PLANTS (Morus sp.)
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Date
2007-09-22
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
Abstract
Present investigation on "stsmdaidization of hardening protocol of tissue
cultured mulberiy plants {Moms spp.)" was carried out to standardize the
hardening protocol for mulberry genotj^es through nodal explants using five
genotypes viz., Vi, S36, (Irrigated); S13, RFS135 (Rainfed) and Mysore local
(Control).
Nodal explants of all the genotypes exhibited survival of 79.36 per cent
when treated with 0,1 per cent HgCb for about 8 minutes. S36 recorded
maximum callus initiation (60.76%) compared to the other genotypes, when
treated with 2.0mg/L 2, 4-D along with 0.75 or l.OOmg/L BAP, 2, 4-D therefore
it was identified as the best auxin for callus induction. Callus differentiation
into shoots was also maximum in S36 variety, when MS medium used was
fortified vdth combination of BAP (2mg/L) and NAA (O.lmg/L). Significantly
higher rooting percentage (48.07%, 40.82%) was recorded when shoots were
transferred to MS media having 1.0 mg/L and 1.5 mg/L NAA respectively as
compared to control (22.50 %). Maximum number of leaves were recorded when
50 per cent and 75 per cent glycerol were applied as antitransparents (4.40.
4.13), followed by low melting paraffin wax (4.00) and 50 per cent grease
petroleum (3.93) compared to control (2.93). Shoot length recorded maximum
when grown on potting mixture (Peat: perlite: vermiculite (1:1:1)+ MS+1 ppm of
NAA+lppm IBA +lppm IAA+(5.46) while sand: soil: perlite fl/2 MS+lppm
lAA+lppm IBA+lppmlAA stood next (5.40). Maximum plant height was
recorded significantly with the 50 per cent shading (3.69%) and 75 per cent
shading (3.42%). Survival rate was recorded significantly higher in mulbeny
with 45 days (81.94%) and 30 days after planting (80.89%). Considering callus
initiation, shoot, internodal length, root formation, plant height, number of
leaves and survival per cent, genot3^e S36 performed best out of all other
varieties tried.
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