Intestinal Stem Cells Isolation and Culture Characteristics in vitro by Morphometry and Flow Cytometry
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Date
2018-05
Journal Title
Journal ISSN
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Publisher
Excellent Publishers
Abstract
Adult stem cells are crucial for maintaining proper function and repair of tissues. The
epithelium of the adult mammalian intestine is a constant dialog with its underlying
mesenchyme to direct progenitor proliferation, lineage commitment, terminal
differentiation and ultimately cell death. The epithelium is shaped into spatially distinct
compartments that are dedicated to each of these events. The unique intestinal
orchestration makes the crypt as one of the most accessible models for the study of adult
stem cell biology (Barker et al., 2007). Because intestine is one of the most rapidly
regenerated tissues in the body, the intestinal crypt has provided an informative system for
studying stem cell biology. The full potential of these models was not realized, however,
duo to the limited availability of optimized protocols and unique stem cell markers to
identify the location and numbers of ICSs as well functional assays to validate the models
in vivo. Though Intestinal stem cell represented by crypt population has been established in
mice and human but the understanding of the underlying mechanism was not reported in
other animal species in detail. The present research focussed on the isolation of intestinal
crypts and their in vitro culture characteristics in chicken, a mammalian animal model
widely used for research and food application has been carried out using cytochemical and
fluorescent activated cell sorting for stem cell specific marker Sox 9.
Description
TNV_Ijcmas_2018_7(5)3731-3735
Keywords
Veterinary Science