MICROPROPAGATION, SOMATIC EMBRYOGENESIS AND GENETIC STABILITY ANALYSIS IN CHRYSANTHEMUM (Dendranthema grandiflora T.) CV. MARIGOLD
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Date
2018-08-03
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K.R.C. COLLEGE OF HORTICULTURE COLLEGE OF ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Abstract
Chrysanthemum (Dendranthema grandiflora T), is the second most important
flower crop and having different colours grown globally. The introduced cultivar
Marigold, which is gaining its popularity in the southern India due to its bright yellow
colour and high shelf- life. It is generally propagated through vegetative means, which
is a time consuming and having low production rate. Present study was undertaken to
standardize the micropropagation and somatic embryogenesis protocol and genetic
stability analysis in order to optimize a better substitution for mass multiplication of
quality planting material and mitigating the demand of farmers as well as consumers.
Nodal segments from the healthy mother plant were sterilized using different surface
sterilants and then cultured in MS and B5 media with different growth regulator
concentrations for shoot initiation, elongation and rooting followed by hardening in
various media. Somatic embryogenesis was undertaken using five different explants
and cultured in MS medium with different concentration growth components as well
as phytohormones.
Among the different media used for micropropagation, B5 media augmented
with 1.5 mg/l BAP was found to be best for shoot bud initiation and showed higher
rate of multiplication. MS medium with 0.5 mg/l kinetin resulted maximum shoot
length whereas B5 medium promoted more number of shoot regeneration. In vitro
root initiation was best in MS media and half strength MS media with 1mg/L IBA
was next best and was on par with MS media. However, half strength media with 1
mg/L IBA was considered more economical.
Among the different explants used for somatic embryogenesis, leaves
produced the best quality callus in MS media containing 1 mg/l 2,4-D, 200 mg/l
Caesin hydrolysate and 290 mg/l L-Proleine. Shoot tips as explants produced the
highest number of shoots per clumps in MS media containing NAA (1.5 mg/l); BAP
(3 mg/l); Casein hydrolysate (150 mg/l) and L-Proline (50 mg/l). Ray florets showed
the earliest callogenesis as well as plant regeneration in each media.
Totally 10 in vitro regenerated plants were randomly selected and tested for
genetic stability using two cultivar specific SRAP primers. Tested micropropagated
plants were 100 per cent similar with field grown mother plants indicating high
efficiency of the optimized micropropagation protocol.
Description
TH-632
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