Assessment of immunogenic potential of Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi
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Date
2018-08
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Typhoid is an important acute systemic illness affecting human beings globally and especially in developing countries of Asia and Africa. The disease leads to millions of deaths and heavy economic loss annually worldwide. The causative agent of typhoid is Salmonella enterica serovar Typhi. Antibiotics for treatment are available but most of them are becoming outdated day by day because of development of multiple drug resistance in Salmonella. Currently available vaccines have their own limitations. Therefore, there is a need to develop better vaccine. Outer membrane proteins have proven their immune potential as well as Calcium phosphate nanoparticles in DNA vaccines as adjuvant have exhibited their potential in provoking efficient cell mediated and humoral immune response. In present study, the calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi (CaP-NPs-Omp complex) were assessed for its efficacy as alternative vaccine candidates for typhoid. The prepared vaccine (CaP-NPsOmp complex) was characterized and tested in mice model. The safety of vaccine was evaluated by toxicity analysis in rats. On characterization of CaP-NPs-Omp complex, particles were found to be in the size range of 20-50 nm with high stability and purity. CaP-NPs-Omp complex produced effective cell-mediated and humoral immune response along with protective immunity. On toxicity analysis, CaP-NPs-Omp complex was found to be safe. On the basis of our findings we could presume that Calcium phosphate nanoparticles encapsulating Omps of S.Typhi may turn out to be an alternative option for a safe, efficient and cost effective vaccine against Salmonella Typhi.
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