The laboratory and field studies were carried out during 2014-15 at Main Agricultural Research Station, UAS, Dharwad to study the influence of bio-priming on seed quality, field performance and morphometric characterization in maize hybrid GH-0727. The laboratory experiment was laid out in Completely Randomized Design and replicated four times. The laboratory experiment consisted of ten treatments. The field experiment consisted of ten treatments and laid out in Randomized Block Design with three replications and another field experiment was conducted to study the morphometric characterization of maize hybrids and parents laid out in Randomized Block Design with three replications. Laboratory studies indicated that hybrid seeds bio-primed with Azospirillum brasilense (20%) and Pseudomonas striata (20%) showed higher germination (97.0% and 96.8%), shoot length (11.10 and 10.98 cm), root length (25.88 and 25.50 cm), vigour index (3587 and 3528), seedling fresh weight (14.69 and 14.60 g), seedling dry weight (2.41 and 2.41 g), catalase activity (0.85 and 0.82 U/min/mg), peroxidase activity(0.76 and 0.75 U/min/mg) and alpha-amylase activity(8.26 and 8.24 µm/min/ml) as compared to other treatments. Results with respect to field experiment revealed that the seed bio-priming with Azospirillum brasilense (20%) recorded higher field emergence (96.3%), plant height (210 cm), number of leaves (12.4), minimum number of days for 50 per cent tasseling and silking (53 and 57.33), higher cob length (18.83cm), cob girth (15.17cm), number of rows per cob (14.67), number of seeds per row (33.73), test weight (30.2 g), grain yield per plot (8.6 kg) and grain yield per hectare (68.28 q). The results of morphometric characterization of maize genotypes showed the variation among parents and hybrids with respect to plant height (long and medium), tassel attitude of lateral branches (straight, curved and strongly curved), tassel angle (wide and narrow), ear shape (conical, cylindrical, conico-cylindrical) and thousand seed weight (large and medium).