Antagonistic effect of Trichoderma for resistance of Fusarium wilt on in vitro grown Grand Nain Banana plant (Musa acuminata)
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Date
2018
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Birsa Agricultural University, Ranchi, Jharkhand-6
Abstract
The antagonistic activity of Trichoderma against Fusarium oxysporum were examined on potato
dextrose agar plates by dual culture method. The results revealed that, all the antagonists showed
inhibitory effect on growth of the test fungus and were effective in controlling the growth of
pathogen. There were two species of Trichoderma, Trichoderma harzianum (2 different strains
viz. 1 and 6) and Trichoderma viride, as there was no specific strains of Trichoderma viride
present. Among the strains of Trichoderma sp. ,TH6 was found to be most effective in inhibiting
the growth of Fusarium oxysporum of about 50 and 72 per cent of test fungus on 5th and 9th day
respectively. The result revealed that both species are effective in antagonizing Fusarium sp. by
mycoparasitic activity and also by competing for both rhizosphere colonization and nutrient.
Plants treated with Trichoderma have altered metabolic activities as they undergo a stress
condition. Thus any changes in the metabolites production was analysed by performing some
biochemical assays. Among them the most effected stress related parameters were anthocyanin
content, proline content, Antioxidative (CAT & SOD) and ROS by Lipid peroxidation activity.
Anthocyanin content was found to be increased significantly upto 10g/F.W. in the T8 treated
plants as compared to control (3.525g/F.W.). Similarly results also reflect increased
accumulation of proline in banana T8 treated plants upto 1.367μM in vitro as compared to control
plants (0.454μM). Also in case of antioxidative enzyme assay, we found that significant 10 fold
increase in CAT and 0.166 Unit mg-1 increase in SOD activity was observed as the enzyme
concentration decrease and increase in ROS was observed in infected plants upto 0.164mM mg-1
as compared to control (0.0358mM mg-1) due to activation of defense mechanism of plant, which
was then maintained on recovery of pretreated infected plants approximately 0.084mM mg-1.On
the basis of these results, T8 treated plant was found to be the most suitable for resisting
Fusarium. Molecular estimation of total protein content was also performed. The quantified
protein so obtained shows an increase in concentration of protein upto 3.327μg/μl in T4 treated
plants as compared to control (1.991μg/μl).
Description
Antagonistic effect of Trichoderma for resistance
of Fusarium wilt on in vitro grown Grand Nain
Banana plant (Musa acuminata)
Keywords
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