Line × Tester analysis and identification of cold tolerant lines in chickpea (Cicer arietinum L.)
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Date
2018-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present investigation was taken up to identify promising parents and crosses through line x tester analysis involving six lines and three testers for grain yield and related traits. Heterosis was also estimated to identify superior combinations. Crosses made in first year, were planted in two replications in next year and were evaluated for ten different agronomic characters. In another experiment, cold tolerant lines were identified based on in vitro techniques. Thirty cultures of chickpea genotypes were incubated in growth chamber at three temperature levels ( 14, 11 and 8 degree Celsius) and data were recorded on four different parameters. In first experiment, analysis of variance was found significant for nine out of ten traits studied and that for combining ability indicated that both the additive and non-additive gene actions were important for expression of the traits. The estimates of σ2 sca were found to be higher than the σ2 gca for all the traits studied except for days to 50 percent flowering, canopy width (cm) and biological yield (g), indicating good prospects for exploitation of non - additive genetic variation for grain yield and components. ICC 26 was ranked as best tester as it had good general combining ability for seven yield attributes including grain yield per plant. Among lines, PG 5 was ranked best as it had good general combining ability for three yield and yield contributing characters. Based on significantly positive sca effects and heterosis over mid, better and check parent, cross combinations PG 5 × ICC 26, PG 186 × ICC 26, PG 3 × ICC 26 , PG 0104 × ICC 26 and PG 170 × ICC 26 were identified as potential crosses for grain yield. In second experiment, the results from analysis of variance revealed significant differences among genotypes and temperature levels. Genotype M 34 appeared to be the best for germination percentage; M 46 for radical length, M 28 and M 30 for plumule length; and IPC-13-89 for fresh weight on the basis of general mean. Genotypes M 28, M 34, M 35, M 36, M 37, M 38, M 39, M 41, M 42 and M 45 were considered better over others for germination percentage with score ‘1’. Similarly, genotypes namely M 28, M 30, M 33, M 34, M 38, M 39, M 40, M 45, M 46, IPC-13-89 and IPC-15-17 for radical length; M 28, M 30, M 35, M 36, M 40, M 43, M 44 and IPC-13-74 for plumule length; and M 29, M 30, M 31, M 33, M 37, M 40, M 45, M 46, IPC-13-89 and IPC-15-17 for fresh weight. Based on non-parametric selection, the genotypes M 28, M 30 and M 40 were found to be cold tolerant with highest score with highest sum score of three.
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