Assessment of genetic diversity in carrot genotypes using morphological, biochemical and molecular markers

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Date
2018
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Punjab Agricultural University, Ludhiana
Abstract
The study entitled “Assessment of genetic diversity in carrot genotypes using morphological, biochemical and molecular markers” was carried out at Vegetable Research Farm, Department of Vegetable Science, Punjab Agricultural University, Ludhiana. Eighty one genotypes of carrot were characterized by morphological, biochemical and molecular markers during 2015-16 and 2016-17. Analysis of variance showed significant differences for 19 quantitative traits with respect to treatment, year and treatment x year. Maximum total root yield was obtained from genotypes viz. PC-161, PC-15, PC-43 and PC-103 (red), PCO-30 and PCO-5 (orange) and PCP-2 and PCP-1 (purple). On the basis of morphological diversity, 81 genotypes were distributed into ten clusters. Cluster X was the best for root weight and anthocyanin content, cluster III for days to 1st root harvest and root girth, while dry matter content, total sugar content and carotene content were maximum in cluster V. The highest intra cluster distance has been observed from the cluster VII (162.9), while inter cluster distance was maximum between II and cluster X (43678.5). The cumulative proportion of variation explained by the four PC-axes was 85.12 %. Seventeen polymorphic SSR markers differentiated genotypes into three groups where cluster I consisted of 48 genotypes followed by cluster II with 28 genotypes and cluster III had 5 genotypes. The morpho-biochemical and SSR markers were effective tools to identify closeness among genotypes, so future strategy involves utilizing more SSR markers culled from various linkage mapping could be effectively and efficiently used to fasten the breeding programs and to design trait specific carrot breeding.
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