A Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus)
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Date
2017-09
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium
Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted
Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and
compared with NCBI reference sequence. The results revealed that OSP had five major
specific proteins bands namely, OSP-I (GATA Zinc finger domain containing protein 1),
OSP-II (GATA Zinc finger domain containing protein 1), OSP- III (E3 ubiquitin protein
ligase RNF 216, OSP-IV (Mitotic spindle assembly checkpoint protein MAD1) and OSPV
(Dual specificity phosphatase DUPD1). The estimated molecular weight of OSP-I
(Mr94.51 to 102.34 kDa), OSP-II (Mr75.19 to 93.07 kDa), OSP-III (Mr59.58 to 72.76
kDa), OSP-IV (Mr30.71 to 40.90 kDa) and OSP-V (Mr21.66 to 26.26 kDa). The results of
our study provide basic knowledge of the protein composition of ostrich seminal plasma
highlighting important physiological pathways which may play crucial roles in the sperm
environment after ejaculation. This knowledge can be the basis to further develop
procedures improving the reproduction of farmed ostrich.
Description
TNV_Ijcmas_2017_6(9)3073-3078
Keywords
Veterinary Science