An experiment was conducted to study the effect of multi-species probiotic on production performance of Japanese Quails. Three hundred Japanese quail day old chicks were randomly divided into a control and three treatment groups with three replicates of 25 each and reared in cages for six weeks. The basal diets were supplemented with multi-species probiotic to control, T1, T2, and T3 diets @ 0, 0.005, 0.01, and 0.02 % level respectively. The parameters such as bi-weekly body weight, FCR, and livability were recorded and analyzed. The groups supplemented with multi-species probiotic at 0.01, and 0.02 % level had resulted in significantly (P < 0.01) higher mean body weight (185.62±2.51g and195.05±2.12g), better FCR (2.84±0.03 and 2.69±0.04) respectively than other groups. It can be concluded that the supplementation of multi-species probiotic in diet at 0.01, and 0.02% levels were found to be beneficial for higher production performance of Japanese quail.