Anion Exchange Chromatography for Purification of Antigen B of Cystic Echinococcosis
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Date
2014-12
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Abstract
Cystic echinococcosis or hydatidosis causes severe economic loss and public health problem to both human
beings and livestock in many temperate and tropical areas of the world. Immuno diagnosis has been found to be
useful not only in primary diagnosis but also for follow up of patients after surgical or pharmacological treatment in
man. Various immune diagnostic tests for hydatidosis in man and animals have been attempted using hydatid cyst
fluid antigens with varied sensitivity and specificity. However these assays using crude hydatid antigens have been
non-specific due to cross reaction with Cysticercus, Coenurus and other helminthic infections. In order to overcome
these difficulties various novel tests using purified antigens are essential for confirmative diagnosis of hydatidosis in
man and animals. One of the subunit of antigen B, 8 kDa protein has been shown to be hydatid specific. Various types
of chromatography have been used for purification of Hydatid antigens. However, Anion exchange chromatography
had been used in this study to get more antigen B 8 kDa than other chromatographic methods
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Keywords
Veterinary Science, Veterinary Parasitology