Bioprospecting Granulosis Viruses (PibrGV) as biopesticide against Pieris brassicae

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Date
2018-10
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu
Abstract
Cabbage white butterfly, Pieris brassicae (L.) (Lepidoptera: Pieridae), is a cosmopolitan destructive pest which can cause extensive damage at all the growing stages of Cole crops such as seedling, vegetative and flowering stages. Investigations were carried out to isolate and bioprospect naturally occurring GVs as potent biopesticides against P. brassicae. Exploratory surveys confirmed the presence of Granulosis virus in the natural population. A total of 3 isolates were isolated from Jammu and Kashmir range of North –Western Himalayas in the present study. Among all these isolates, Sudhmahadev isolate of PibrGV was found to be most effective because of its higher viral activity and speed of kill against all the instars tested. Significant differences were observed in larval development, per cent pupation and adult emergence, larval and pupal weight when compared with control. However, no significant difference was observed in the sex ratio of adults emerged from treated larvae as compared to control. The PCR amplification of genomic DNA with specific primers confirmed the presence of Pieris brassicae granulovirus (PibrGV) DNA in infected P. brassicae larvae. When the most virulent isolates were subjected in vivo mass production for the highest productivity and quality, it was found that optimum yield was obtained when early 3rd instar P. brassicae larvae were inoculated with the dose of 7.2 × 104 OBs/larva and allowed to incubate for 12 days incubation period. To prevent microbial contamination moribund larvae were harvested despite the loss of OBs. In the laboratory pot experiment PibrGV + Lepidium + Jaggery was found to be the most superior while PibrGV + Teepol + Zno + Tio2 was found to be the least superior among all the treatments. However, in field experiments Spinosad and PibrGV + Bacillus thuringenesis were found to be most effective treatments in reducing the larval population of P. brassicae followed by PibrGV + Lepidium + Jaggery and PibrGV + NSKE. This suggests that the Sudhmahadev isolate of PibrGV is likely better suited for the development as a biological insecticide for the management of P. brassicae larval populations in J & K as a promising viable alternative to chemical insecticides.
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