In vitro studies on anther culture development of sunflower (helianthus annuus L.) genotypes
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Date
2018-05-30
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Sunflower (Helianthus annuus L.), is a multi-purpose crop employed for diverse uses such as dye production, edible oil extraction and for medicinal applications. Production of microspore-derived embryos from cultured anthers is now well established technique for isolation of homozygous lines in sunflower. The present investigation deals with In vitro haploid plant production in sunflower and confirmation of haploids by molecular markers. Treatment 2.0mg/l NAA+1.0mg/l BAP was superior over rest of the treatments and gave maximum response for callus induction (90%) in Morden. The best shoot induction response was recorded in TSG-63 (21.80 %) in treatment 0.5 mg/l BAP and the best shoot elongation response was recorded in EC-16400 (70%) in treatment 0.2 mg/l BAP.
The regenerated haploid callus shoots and its donor parents were screened with 28 SSR markers for haploid confirmation. Seven SSR primers viz., ORS-05, ORS-662, ORS-1265, ORS-484, ORS-488, ORS-598 and ORS-811 were found useful for the identification of haploids in sunflower. The banding patterns of the markers appeared in the antheral calli lines, regenerated line and the donor parent line. Both the regenerated haploid lines had homozygous profiles with a single band while the donor parent had two bands that indicated allelic sites. The seven SSR markers showed 100% homozygosity. The outcome of the molecular analysis and evaluation of haploid confirmation provide useful information for the development of sunflower breeding.
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