In silico sequence analysis and molecular phylogeny of DCL, AGO and RDR proteins in legume crops
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Date
2018-09
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OUAT
Abstract
Biotic stress in legume crops is one of the major threats to crop yield and
productivity. RNA silencing is an evolutionary conserved mechanism that regulates
variety of cellular process in plants. Plants have evolved some control mechanisms that
efficiently prevent pathogen invasion to protect themselves from pathogen attack
throughout their life-cycles. Dicer-like (DCL), Argonaute (AGO), and RNA-dependent
RNA Polymerase (RDR) proteins are the key components of RNA silencing and are
known to play a significant role in combating biotic and abiotic stresses. In this study,
we focused on identification of AGO, DCL and RDR genes in 8 important legume crops
namely green gram, red gram, Groundnut, pigeonpea, chickpea, soyabean and wild
soyabean. A total of 21 DCLs, 28 RDRs and 42 AGOs were found to be conserved
across all the 8 legumes. These proteins are particularly participating in regulation of
developmental process like leaf and flower development as well as conferring
resistance to pathogens. The conserved motifs and domain of these genes are identified
and used for phylogenetic analysis that classified DCL, RDR and AGO gene family
into four clades.
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