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|Advisor:||Singh, Dr. O.P.|
|Title:||Effect of Arsenic Levels on Germination Attributes and Biochemical Parameters in Seedlings of Wheat (Triticumaestivum)|
|Publisher:||DEPARTMENT OF PLANT PHYSIOLOGY INSTITUTE OF AGRICULTURAL SCIENCES BANARAS HINDU UNIVERSITY VARANASI-221005 INDIA|
|Keywords:||Arsenic, Wheat,proline, phytotoxicity, plumule.|
|Abstract:||The present study entitled“Effect of Arsenic Levels on Germination Attributes and Biochemical Parameters in Seedlings of Wheat (Triticumaestivum)” with two wheat genotypes (Sonalika and HUW 234),five levels of arsenic (Na2HAsO4.7H2O @ 0.0mM i.e. control, 0.5mM, 1.0mM, 3.0mM and 5.0mM) and three replications in large petri plateswas carried out in the Laboratory of Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University during 2016-17. The experiment was carried out to investigate the effect of different doses of arsenic on changes in germination percentage, radicle length, plumule length, seedling vigor index, percent phytotoxicity, metal tolerance index, effect of arsenic stress on seed germination (percent difference from control), total protein content, total sugar content, proline content in all treatments in 7 days old seedlings. The design followed wasFactorial experiment in CRD (FCRD) and standard statistical procedures were employed for analysis. It can be concluded from the above experiment that heavy metal arsenic had profound effect on germination attributes and biochemical parameters in both wheat genotypes. Per cent germination, radical length, plumule length, seedling vigor index, metal tolerance index, total soluble sugar and protein content decreased significantly, whereas, per cent phytotoxicity and proline content increased significantly with increasing toxicity of arsenic.Wheat genotype,HUW 234 was found to be more tolerant to heavy metal arsenic in comparison towheatgenotype, Sonalika as evidenced by lesser per cent phytotoxicity and higher proline content, which might reduce the heavy metal stress, attributing to better germination and growth of seedlings. Arsenic toxicity of up to 0.5 mM was not much harmful for germination and seedling growth in wheat. The drastic reduction in germination, growth and biochemical parameters were observed atNa2HAsO4.7H2O @ 1.0mM and above concentrations.|
|Appears in Collections:||Thesis|
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