Molecular mapping of nuclear male sterility gene ms-1 in muskmelon (Cucumis melo L.)
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Date
2018
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Punjab Agricultural University, Ludhiana
Abstract
Nuclear male sterility (NMS) is one of the most extensively exploited pollination control
mechanisms for hybrid breeding in muskmelon. Punjab Agricultural University, Ludhiana has
developed three hybrids, Punjab Hybrid, Punjab Anmol, MH-27 using MS-1 male sterile
genotype possessing ms-1 gene. Male sterility reduces the cost of hybrid seed production, but
the 50% heterozygous male fertile plants in the female line need to be rouged out before
pollination. A codominant molecular marker closely linked to ms-1 gene would not only
facilitate its rapid transfer to new inbred lines, but it will also assist the removal of 50 %
fertile plants in the female line before transplanting in the hybrid seed production block.
Therefore, the present study was undertaken to identify codominant simple sequence repeat
(SSR) marker(s) linked to ms-1 gene in an F2 population derived from a cross ‘MS-1 ×
KP4HM-15’.Segregation analysis of F2 population confirmed the monogenic recessive
inheritance of the male sterility gene ms-1. Total 498 SSR primers were used for analysis of
polymorphism between the parents followed by bulk segregant analysis (BSA). The primers
differentiating the parents as well as the sterile and fertile bulks were further used for
screening of F2 population to assess the linkage distance between the ms-1 gene and the
putative markers. It was found that two SSR markers, DM0187 and DM0038 were linked to
the ms-1 gene. The marker, DM0187 was closely linked at a genetic distance of 6.6 cM while
the distance of ms-1 locus and DM0038 marker was 21.1 cM. Linkage analysis placed ms-1
locus between the two marker loci on chromosome 6 of muskmelon. The closely linked
marker, DM0187 can be used to speed up the transfer of ms-1 gene into new desired lines. It
will also aid the elimination of fertile plants in the female line at seedling stage. Further, it
provides perspective for the fine mapping and cloning of ms-1 gene.
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