Assessment of genetic variability through morphological traits and seed protein profiling in ridge gourd [Luffa acutangula (Roxb.)]
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Date
2014-06-05
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College of Horticulture and Forestry, Central Agricultural University, Pasighat
Abstract
Analysis of variance revealed significant differences among the genotypes for all the attributes. The genotypes CHRG-10, CHFRG-22, CHFRG-28, and CHFRG-30 were found to be superior for the most of the yield components and fruit quality traits. High PCV and GCV, heritability, genetic gain were observed for vine length, number of node per vine, number of node to first pistilate flower appearance, pedicel length, fruit length, number of fruit per plant, and yield per plant.
Correlation studies indicated that fruit yield per plant was positively and significantly correlated with vine length, number of node per vine, crop duration, fruit length, fruit diameter, number of fruit per plant and average fruit weight. However, negative and significant association was established with days to first staminate flower anthesis and pistillate flower anthesis.
Maximum positive direct effect on fruit yield per plant was imposed by number of fruit per plant, vine length, average fruit weight, fruit length, days to first staminate flower anthesis, days to first pistilate flowerer anthesis and days to 50% emergence which were observed as the most important traits affecting fruit yield per plant. However, high negative direct effect was observed for number of node per vine, intermodal length and fruit diameter.
Divergence study revealed that days to first staminate flower anthesis contributed maximum percent to diversity followed by number of fruits per plant, days to first fruit harvest, fruit length, fruit diameter and pedicel length. Maximum inter cluster distance was observed between cluster IV and VI and indicated that the genotypes within these were highly divergent.
SDS-PAGE analysis showed a total of 93 bands identified by silver staining. The genotypes exhibited considerable variation in protein band number ranging from 8-24. Among the genotypes CHFRG-11 showed maximum number (24) of protein bands while the minimum numbers (8) of bands were present in CHFSM-12.
Description
The present investigation was conducted during summer 2013 at Vegetable experimental farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh in 40 diverse genotypes of ridge gourd. The experiment was laid out in randomized complete block design with three replications. Genetic variability was studied for attributes viz., days to 50% emergence, vine length, number of node per vine, intermodal length, crop duration, number of node to first pistilate flower appearance, days to first staminate flower anthesis, days to first pistilate flower anthesis, days to first fruit harvest pedicel length, fruit length, fruit diameter, number of fruits per plant, average fruit weight and fruit yield per plant. Data were analyzed statistically for phenotypic and genotypic variance. Coefficient of variation, hereditability, genetic advance, genetic gain, correlation, coefficient, path coefficient, genetic divergence and seed protein banding pattern.
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