Development of continuous microwave pasteurization system for liquid foods

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Date
2017
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DIVISION OF FOOD SCIENCE & POSTHARVEST TECHNOLOGY ICAR - INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI –
Abstract
A continuous pasteurization system for liquid foods was developed. The developed system used microwave power to heat the liquid food instead of conventional heating. Preliminary experimentation to obtain a time-temperature combination for complete inactivation of B. cereus and S. cerevisiae using nutrient and MGYP broths respectively was undertaken using conventional heating method. The developed system was subsequently tested for its efficacy in reducing microbial load (B. cereus and S. cerevisiae) in model liquid system at 90 and 60°C respectively. It was found that microwave heating could completely eliminate the microbial load in each of the two liquid systems in significantly lower residence times (3 min. instead of 8 min. for B. cereus and 5 min. instead of 7 min. for S. cerevisiae) compared to those using conventional heating. The developed continuous microwave pasteurization system tested for pasteurization of sugarcane juice and soy milk using different combinations of microwave power levels (210, 280, 350, 420 & 490 Watts) for heating the liquids to 70°C and 100°C respectively. The heated liquids were then held for 10 min. and 1 min. respectively at 70° and 100°C, cooled, qualitative evaluation done and storability studied under refrigerated condition (4±1°C). Quality evaluation of the Pasteurized sugarcane juice and soy milk showing maximum retention of total antioxidant content was subsequently stored under refrigerated conditions (4±1ºC) in transparent glass bottles. Colour (L*, a*, b*) values of fresh sugarcane juice added with citric acid, thermal pasteurized and microwave pasteurized juices were significantly higher from that of fresh sugarcane juice. There was no significant difference in TSS and pH values of any of the pasteurized sugarcane juice. PPO activity and total viable bacterial count decreased significantly after thermal and microwave pasteurization of juice whereas yeast was completely eliminated from all the microwave pasteurized and thermal pasteurized sugarcane juices. There was a significant decrease in total antioxidant values after thermal and microwave pasteurization whereas sensory scores of fresh sugarcane juice added with citric acid did not change significantly after thermal and microwave pasteurization. 99 There was no significant difference in colour (L*, a*, b*), T.S.S. and pH values between the fresh, thermal pasteurized and microwave pasteurized soy milk. Total antioxidants, total phenols, LOX activity and total viable bacterial count in freshly prepared soy milk decreased significantly after thermal and microwave pasteurization. There was no significant difference in Colour and texture of Soy milk after microwave and thermal pasteurization whereas beany flavour, taste and overall acceptability scores changed significantly after microwave and thermal pasteurization. During storage there was no significant difference in colour (L*, a*, b*) and TSS values of microwave pasteurized sugarcane juice at 490 Watt and thermal pasteurized (control) sugarcane juice throughout the storage period.There was a significant decrease in pH, residual PPO activity and total antioxidant content in microwave pasteurized and thermal pasteurized sugarcane juice with the increase in storage period.There was a significant increase in total viable bacterial count in microwave pasteurized and thermal pasteurized sugarcane juice at the end of storage whereassensory scores of microwave pasteurized and thermal pasteurized sugarcane juice decreased gradually during storage. During storage there was no significant difference in colour (L*, a*, b*) values of microwave pasteurized soy milk at 420 watt throughout the storage period where as colour (L*, a*, b*) values for thermal pasteurized (control) soy milk changed significantly at the end of storage. There was no significant difference in TSS values of microwave pasteurized and thermal pasteurized soy milk throughout the storage period. There was a decrease in pH, residual LOX activity, total antioxidants and total phenols content in microwave pasteurized and thermal pasteurized soy milk with the increase in storage period. There was a significant increase in total viable bacterial count in microwave pasteurized and thermal pasteurized soy milk at the end of storage whereassensory scores of microwave pasteurized and thermal pasteurized soy milk decreased gradually during storage. Microwave technology can be used successfully for the pasteurization of liquid foods with higher retention of nutritional and functional quality of liquid foods.
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