In vitro studies on the variations of biochemical metabolites in Glycyrrhiza glabra by using various elicitors
Loading...
Files
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Biochemistry & Biochemical Engineering, Jacob Institute of Biotechnology & Bioengineering, Sam Higginbottom University of Agriculture, Technology & Sciences Allahabad-211007
Abstract
The Glycyrrhiza glabra, of the Fabaceae family, is a medicinal and edible herbs that contain a
wide range of phytochemicals which are used pharmaceutically and commercially. Glycyrrhiza
glabra is under the threat of overexploitation and depletion therefore, there is an urgent need for
conservation. It is advantageous to develop in vitro techniques not only for propagation,
multiplication and preservation but also for elicitation of secondary metabolite production. The
present study provides information on the micropropagation of G. glabra related to the use of
different explants, the combination of plant growth regulators with different sterilization
strategies, the culture conditions, and additional factors influencing in vitro propagation (such as
light, temperature, humidity and pH). Conservation of germplasm from G. glabra, through
encapsulation have also been studied which avail the germplasm for commercial cultivation over
the long run. Qualitative and quantitative screening for phytoconstituents and the evaluation of
their antimicrobial activity have also been presented. Enhanced production of glycyrrhizin, main
bioactive component of G. glabra by different elicitors is deliberated. Successful multiplication
and elicitation will lead to the production of not only greater quantities of planting material with
improved quality but also commercially desired metabolites. This study will be helpful in future
studies on somaclonal variation, genetic transformation and drug discovery.
Description
Ph. D. thesis
Keywords
null