In vitro studies on mass propagation and secondary metabolite enhancement in two important medicinal plants: Allium sativum var. Snow mountain garlic and Lavatera cashmeriana (an endangered medicinal plant)
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Date
2018
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Department of Biological Sciences Sam Higginbottom University of Agriculture, Technology and sciences (Formally Allahabad Agricultural Institute) Allahabad-211007
Abstract
The genus Allium and Lavatera includes hundreds of medicinal plant species, serves human beings in both therapeutic and culinary purposes and has attained its position amid the vast and most essential representative genus of Alliaceae and Malvaceae families respectively. Allium sativum and Lavatera cashmeriana are mostly known by their rich content of flavonols, steroidal saponins, fructans, antioxidant enzymes etc. The present study focuses on the use of biotechnology for in vitro culture, conservation and elicitation of secondary metabolite content ofLavatera cashmerianaandAllium sativum. In vitro regeneration using explant and plant growth regulator combinations with different sterilization strategies have been reported in this study. Tissue culture technology is potent for accelerated growth, multiplication and production of pathogen free healthy plants and has opened wide areas of research for biodiversity conservation. Different abiotic elicitors (Chemical elicitors: Biotin, Adenine sulphate, Salicylic acid; Polyamines: Spermine, Spermidine, Putrescine; Nano particles: Iron NP, Silver NP, Copper NP, Zinc NP) were incorporated in the study to enhance metabolite content in plants. Among chemical elicitorsAdenine sulphate was the efficient in up regulating the metabolite content in Lavatera while in Allium biotin caused maximum increase in metabolite contents. In case of polyamines Putrescine was remarkably efficient in causing increase in metabolite contents of both the plants. Nano particles also resulted in enhancement of metabolite contents in both the plants howeversilver NP and iron NP causes maximum increase inLavateraand Allium respectively.
Description
Ph.d. thesis
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