Genetic Diversity and Seed protein electrophroresis in Chilli (Casicum Annuum L.)

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Date
2012-12-29
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College of Horticulture and Forestry, Central Agricultural University, Pasighat
Abstract
Analysis of variance revealed significant differences among the genotypes for all the characters. The genotypes Viz., CHFC-1, CHFC-7, CHFC-8, CHFC-13, CHFC-14, CHFC-25 and CHF-C26 were promising as they had more that one desirable traits. High PCV and GCV, heritability, gnetic gain were observed for days to first flowering , plant height, number of seed per fruit, number of fruit per plant, ascorbic acid and fruit yield jper plant. Correlation studied indicated that fruit yield per plant was positivsely and significantly correlated with number of branch per plant, number of ffrujit per plant and chlorophyll content but negatives and significant association was established with ascorbic acid content. Maximum positive direct effect on fruit yield per plant was impose by fruit weight, number of fruit per plant, number of seed per fruit and capsaicin content. However, high negatives direct effect was observed for fruit diameter, fruit length, number of primary branch per plant and plant height. Divergence study revealed that capsaicin content contributed maximum percent to diversity followed by ascorbic acid content, chlorophyll content, fruit weight, number of fruit per plant, green fruit yield per plant and fruit length. :Maximum intercluster distance was observed between cluster 2 and 4 and indicated that the genotypes within these were highly divergent. SDP-PAGSE analysis showed considerable variation in band number of protein which ranged from 7-19. Protein banding profile showed that genotypic CHFC11 was grouped with genotypes CHFC-12 and CHFC-13 and genotype CHFC-26 was group0ed with genotypes CHFC-25 and CHFC-27. As per morphological and protein dendrogram chilli genotypes; CHFC-16, CHFC-17 and CHFC-19 and genotypes CHFC-22, CHFC-24 and CHFC-30 were placed in one major cluster. SDS-PAGE marke data provided more sub groupings and revealed higher amount of diversity as compared to morphological data in present study.
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The present investigation was conducted during Kharif 2011 at Vegetable Research Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Aruanchal Pradesh in 30 diverse genotypes of chilli. The experiment was laid out in Randomized Complete Block Design with three replications. Genetic Variability was studied for characters viz., plant height, number of primary branch per plant, days to first flowering, fruit length, fruit diameter, number of seed per fruit, number of fruit per plant, fruit weight, capsaicin content, ascorbic acid content, chlorophyll content and green fruit yield per plant Dara were analyzed statistically for phenotypic and genotypic variance, coefficient of variation, heritability, genetic advance, genetic gain, correlation coefficient, path coefficient, genetic divergence and seed protein banding pattern
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